Phytochemically Synthesized Silver Nanoparticles using Ocimum Basilicum Leaf Extract Potentiate Cytotoxicity

A study was conducted to develop a cost-effective and eco-friendly method for synthesizing silver nanoparticles (AgNPs) using Ocimum Basilicum leaf extract (OBLE). The physical and chemical properties of the synthesized AgNPs were analyzed. The X-ray diffractogram of OBLE-mediated AgNPs (OBLE@AgNPs) exhibited face-centered cubic crystallinity with space group Fm3m, which was confirmed by Rietveld refinement studies. Fourier Transform Infrared (FTIR) spectroscopy identified the functional groups of the phytochemicals present in OBLE at 3416 cm(-1) and 1652 cm(-1), indicating the presence of phenolic (-OH) content belonging to OBLE. The formation of AgNPs was confirmed by a characteristic hump at 403 nm in the UV-Vis spectrum. The granular microstructure of OBLE@AgNPs showed wellinterlinked grains with agglomeration and an average grain size of 22 nm. The anticancer activity of OBLE@AgNPs was evaluated against MDA-MD 231 breast cancer cell lines, and the results indicated dose-dependent cell viability with an IC50 value of 67.83 mu g/mL. The present study demonstrated a simple and environment-friendly method for synthesizing AgNPs using OBLE, which could potentially be used in various biomedical applications. [GRAPHICS]