Characterization of the catalytic ability and surface properties of a heterogeneous biocatalyst obtained by the sol-gel method

被引:3
|
作者
Rybochkin, P., V [1 ]
Kamanina, O. A. [1 ]
Lantsova, E. A. [1 ]
Arlyapov, V. A. [1 ]
Saverina, E. A. [2 ]
机构
[1] Tula State Univ, Lenin Pr 92, Tula 300012, Russia
[2] Russian Acad Sci, Zelinsky Inst Organ Chem, Leninsky Pr 47,Str 1, Moscow 119991, Russia
关键词
YEAST DEBARYOMYCES-HANSENII; SILICA; IMMOBILIZATION; BIOSENSOR; BOD;
D O I
10.1007/s10971-022-05915-9
中图分类号
TQ174 [陶瓷工业]; TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
A heterogeneous biocatalyst was obtained by immobilizing the cells of the yeast Debaryomyces hansenii VKM Y-2482 and the bacteria Paracoccus yeei VKM B-3302 into an organosilica material using the sol-gel method. The catalytic activity of immobilized cells was characterized using a heterogeneous biocatalyst as a bioreceptor element of the biosensor. The surface properties of the synthesized material were studied using the BET and BJH methods. It was shown that a heterogeneous biocatalyst with a composition of 50 vol.% MTES and 50 vol.% TEOS exhibited the highest catalytic efficiency. The formed material was mesoporous with slit-like pores. It was shown that the BOD-biosensor with the developed heterogeneous biocatalyst allows data to be obtained that were consistent with the certified method for determining the BOD. [GRAPHICS] .
引用
收藏
页码:310 / 319
页数:10
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