Production, physico-chemical properties and antioxidant activity of melanin from Annulohypoxylon stygium (Lév.) YM Ju, JD Rogers and HM Hsieh

被引：0

作者：

Liu, Ruofan ^{[1
]}

Mo, Cuiyuan ^{[1
]}

Meng, Xianfu ^{[1
,2
]}

Wei, Xuetuan ^{[1
,2
]}

Ma, Aimin ^{[1
,3
]}

机构：

[1] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan, Peoples R China

[2] Huazhong Agr Univ, Key Lab Environm Correlat Dietol, Minist Educ, Wuhan, Peoples R China

[3] Minist Agr & Rural Affairs, Key Lab Agromicrobial Resources & Utilizat, Wuhan, Peoples R China

来源：

NATURAL PRODUCT RESEARCH | 2024年

基金：

中国国家自然科学基金;

关键词：

Optimisation; 3; 4-dihydroxyphenylalanine melanin; HepG2; cells; antioxidant enzyme activity; alcoholic liver injury; STRUCTURAL-CHARACTERIZATION; AURICULARIA-AURICULA; NATURAL MELANIN; LACHNUM YM226; FUNGUS; MUSHROOM; PIGMENT;

D O I：

10.1080/14786419.2024.2320741

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

To obtain higher melanin production in liquid culture, culture conditions of Annulohypoxylon stygium (Lev.) Y.M. Ju, J.D. Rogers and H.M. Hsieh were optimised. The results showed that using single factor experiment and orthogonal test, the optimised production of melanin reached 2.20 g/L, which was 2.06 times higher than that of the control group. In addition, it was speculated that A. stygium melanin (AsM) was 3,4-dihydroxyphenylalanine (DOPA) melanin and showed an amorphous irregular structure. Moreover, it had good solubility in alkaline solution. AsM showed good antioxidant activity at a concentration of 500 mg/L, with DPPH, ABTS and OH radicals scavenging activities of 90.83%, 75.36% and 70.90%, respectively. AsM prevented alcohol-induced oxidative damage and oxidative stress in HepG2 cells by inhibiting the decrease of antioxidant key enzyme activity under alcohol stimulation. It was proved to have a great potential for application as a natural antioxidant and a substitute for synthetic pigments. {Graphical Abstract}

引用

页数：10