Genetic testing and microstructure characterization of biological crusts on the rammed soil surface at the Shanhaiguan great wall in China

Protection of cultural relics and sites is of great significance. In this study, the new gray-green thin-layer biological crust on the rammed soil surface at the Shanhaiguan Great Wall in China was found. The emergence of this material has substantially improved the resistance of the rammed Earth Great Wall to rain erosion. 16S rRNA gene sequencing on the surface crusts of rammed Earth was performed. Results show the biological crusts were mainly algae-based composite crusts containing fungi. At the genus level, microalgae and Sphingomycetes were predominant. Under scanning electron microscopy (SEM), algae filaments dominated by filamentous algae overlapped and intertwined with each other. Furthermore, polysaccharide organic matter secreted by algae formed a covering film. The two formed a complex spatial network structure to envelop soil particles, which enhances erosion resistance. The conformable biological crusting is expected to be used as a new civil engineering material for the protection of rammed Earth sites in the future.