Effects of trace elements on digester performance and microbial community response in anaerobic digestion systems

Trace elements (TE), as micronutrients for microorganisms, have a significant impact on the stability of anaerobic digestion (AD). Studies have been conducted on process stability and performance of the AD of food waste (FW) by supplementing TEs. In this study, mesophilic batch biomethane potential (BMP) tests using FW were conducted to investigate the effect of TEs (Fe, Ni, Co, Se, and Mo) as single and mixed ions. In view of their scarcity, correlations between the microbial community and digester performance such as first-order hydrolysis coefficient (K-h), volatile fatty acids (VFA), methane yield, and methane production rate (MPR) have been developed. Ni2+ at 1 and 1.5 mg/L increased the methane yield by 27% and 23% respectively. Similarly, Co2+ at 0.1 and 0.5 mg/L increased the yield by 21% and 23% respectively, compared to control. Although Se4+ at all concentrations enhanced the methane yield, Fe2+ at only 50 mg/L increased methane yield by 22%. For mixed TEs, the combination of Ni2+ [1 mg/L] +Co2+ was the best and increased methane for all Co2+ concentrations (0.1, 0.4 and 0.5 mg/L) by 16%, 14% and 12% respectively. Firmicutes and Methanosaeta were the most abundant phyla among hydrolytic and methanogenic microbial groups, respectively, constituting 42%-61% and 60-80% of their respective microbial groups. The most significant positive correlations were observed between aceto/acidogenic microorganisms and final VFA concentrations with Pearson correlation factors of 0.91.