Comparison of the Efficiency of Hyperspectral and Pulse Amplitude Modulation Imaging Methods in Pre-Symptomatic Virus Detection in Tobacco Plants

被引：3

作者：

Grishina, Alyona ^{[1
]}

Sherstneva, Oksana ^{[1
]}

Zhavoronkova, Anna ^{[1
]}

Ageyeva, Maria ^{[2
]}

Zdobnova, Tatiana ^{[1
]}

Lysov, Maxim ^{[1
]}

Brilkina, Anna ^{[2
]}

Vodeneev, Vladimir ^{[1
]}

机构：

[1] Natl Res Lobachevsky State Univ Nizhny Novgorod, Dept Biophys, 23 Gagarin Ave, Nizhnii Novgorod 603950, Russia

[2] Natl Res Lobachevsky State Univ Nizhny Novgorod, Dept Biochem & Biotechnol, 23 Gagarin Ave, Nizhnii Novgorod 603950, Russia

来源：

PLANTS-BASEL | 2023年 / 12卷 / 22期

关键词：

Nicotiana benthamiana; biotic stress; potato virus X; pre-symptomatic detection; chlorophyll fluorescence imaging; hyperspectral imaging; CHLOROPHYLL FLUORESCENCE; DISEASE DETECTION; PATHOGEN; INFECTION; MOVEMENT;

D O I：

10.3390/plants12223831

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Early detection of pathogens can significantly reduce yield losses and improve the quality of agricultural products. This study compares the efficiency of hyperspectral (HS) imaging and pulse amplitude modulation (PAM) fluorometry to detect pathogens in plants. Reflectance spectra, normalized indices, and fluorescence parameters were studied in healthy and infected areas of leaves. Potato virus X with GFP fluorescent protein was used to assess the spread of infection throughout the plant. The study found that infection increased the reflectance of leaves in certain wavelength ranges. Analysis of the normalized reflectance indices (NRIs) revealed indices that were sensitive and insensitive to infection. NRI700/850 was optimal for virus detection; significant differences were detected on the 4th day after the virus arrived in the leaf. Maximum (F-v/F-m) and effective quantum yields of photosystem II (Phi(PSII)) and non-photochemical fluorescence quenching (NPQ) were almost unchanged at the early stage of infection. Phi(PSII) and NPQ in the transition state (a short time after actinic light was switched on) showed high sensitivity to infection. The higher sensitivity of PAM compared to HS imaging may be due to the possibility of assessing the physiological changes earlier than changes in leaf structure.

引用

页数：16