Environmental assessment of sewage contamination in the surroundings of a marine outfall combining human mastadenovirus and fecal indicator bacteria

被引：1

作者：

de Macena, Lorena da Graca Pedrosa ^{[1
]}

Feitosa, Renato Castiglia ^{[2
]}

da Silva, Jansen Couto ^{[1
]}

Ferreira, Fernando Cesar ^{[1
]}

Maranha, Adriana Goncalves ^{[1
]}

Branda, Marcelo Luiz Lima ^{[3
]}

Caldeira, Nathalia Goncalves Santos ^{[4
]}

Couto, Jessica Soldani ^{[4
]}

de Azevedo, Mariana Goncalves Coelho ^{[4
]}

de Paula, Bruna Barbosa ^{[1
]}

Miagostovich, Marize Pereira ^{[1
]}

机构：

[1] Oswaldo Cruz Fdn FIOCRUZ, Oswaldo Cruz Inst IOC, Lab Comparat & Environm Virol, Ave Brasil 4365,Pavilhao Helio & Peggy Pereira, BR-21040360 Rio De Janeiro, Brazil

[2] Oswaldo Cruz Fdn FIOCRUZ, Natl Sch Publ Hlth ENSP, Dept Sanitat & Environm Hlth, Rua Leopoldo Bulhoes 1-480, BR-21041210 Rio De Janeiro, Brazil

[3] Oswaldo Cruz Fdn FIOCRUZ, Inst Technol Immunobiol Biomanguinhos, Ctr Adm Vinicius Fonseca Manguinhos, Dept Qual Control,Lab Microbiol Control, Ave Brasil 4365, BR-21040900 Rio De Janeiro, Brazil

[4] Oswaldo Cruz Fdn FIOCRUZ, Natl Inst Qual Control Hlth INCQS, Dept Microbiol, Lab Food & Sanitizes, Ave Brasil 4-365, BR-21040900 Rio De Janeiro, Brazil

来源：

MARINE POLLUTION BULLETIN | 2023年 / 193卷

关键词：

Foreshore; Off-the-coast; Water quality; HAdV; FIB; PMAxx-qPCR; HUMAN ADENOVIRUSES; ENTERIC VIRUSES; DRINKING-WATER; QUANTIFICATION; SEDIMENT;

D O I：

10.1016/j.marpolbul.2023.115110

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

This study assessed the microbiological contamination of the marine area of a metropolitan region, where a marine outfall is used as a sanitary solution for domestic sewage. For human mastadenovirus (HAdV) quanti-fication 134 water samples were concentrated by skimmed milk flocculation method and analyzed with qPCR and PMAxx-qPCR, being the latter to assess the capsid integrity viral. HAdV with intact capsids were detected in 10 % (16/102) of samples classified as suitable for bathing using at least one fecal bacterial indicator. Spatial analysis of the results showed that the drainage channels of the basin that flow into the sea are the main sources of microbiological contamination in the foreshore zone, where intact HAdV reached a concentration of up to 3 log genomic copies/L. HAdV serotypes A12, D, F40 and F41 were characterized. Our results suggest the use of intact HAdV as a complementary parameter to assess the quality of recreational waters.

引用

页数：10

共 16 条

[1] Decay of sewage-sourced microbial source tracking markers and fecal indicator bacteria in marine waters
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