Identification of Tumor Suppressor Gene LHPP-Based 5-microRNA Signature That Predicts the Early- and Midstage Esophageal Squamous Cell Carcinoma: A Two-Stage Case-Control Study in the Chinese Han Population

被引:1
|
作者
Zhao, Xiang [1 ]
Zhu, Xiaocun [2 ]
Wang, Luoshai [3 ]
Chen, Yurao [1 ]
Chen, Ronghuai [1 ]
Zheng, Zemao [1 ]
Yang, Hengjin [1 ]
Xia, Wan [1 ]
Yao, Juan [1 ,4 ]
Zhao, Kun [5 ]
机构
[1] Huaian Hosp Huaian City, Dept Radiat Oncol, Huaian, Peoples R China
[2] Huaian Hosp Huaian City, Dept Gen Surg & Breast Surg, Huaian, Peoples R China
[3] Huaian Hos pital Huaian City, Dept Cardiothorac Surg, Huaian, Peoples R China
[4] Nantong Univ, Taizhou Peoples Hosp, Dept Oncol, Taizhou, Peoples R China
[5] Huaian Hosp Huaian City, Dept Oncol, Huaian, Peoples R China
关键词
next-generation sequencing; esophageal squamous cell carcinoma; microRNA; logistic regression model; biomarker; diagnosis; PROLIFERATION; PROGNOSIS; INVASION;
D O I
10.1093/labmed/lmac125
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Objective To establish a novel approach for diagnosing early- and midstage esophageal squamous cell carcinoma (ESCC). Methods The tumor suppressor gene phospholysine phosphohistidine inorganic pyrophosphate phosphatase (LHPP)-based miRNA signature was identified using next-generation sequencing and 3 biological online prediction systems. This retrospective study established and validated an ESCC prediction model using a test cohort and a validation cohort. Results Immunohistochemical staining and real-time quantitative polymerase chain reaction (RT-qPCR) results showed that LHPP protein levels were significantly lower in tissues with early- and midstage ESCC than in adjacent tissues (P < .01). Further, we confirmed that miR-15b-5p, miR-424-5p, miR-497-5p, miR-363-5p, and miR-195-5p inhibited LHPP. These 5 miRNAs were significantly elevated in the plasma of early- and midstage ESCC (P < .05). An ESCC prediction model combining these 5 miRNAs was established. Finally, in the external validation cohort, the model exhibited high discriminative value (sensitivity/specificity: 84.4%/93.3%). Conclusions The prediction model has potential implications for diagnosis of early- and midstage ESCC.
引用
收藏
页码:411 / 423
页数:13
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