Evaluation of Antioxidant Effects of Pumpkin (Cucurbita pepo L.) Seed Extract on Aging- and Menopause-Related Diseases Using Saos-2 Cells and Ovariectomized Rats

被引：3

作者：

Oh, Joohee ^{[1
]}

Hong, Sookyeong ^{[1
]}

Ko, Seong-Hee ^{[1
]}

Kim, Hyun-Sook ^{[1
]}

机构：

[1] Sookmyung Womens Univ, Coll Human Ecol, Dept Food & Nutr, Seoul 04310, South Korea

来源：

ANTIOXIDANTS | 2024年 / 13卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

antiaging; menopause; pumpkin seed; oxidative stress; antioxidants; cardiovascular diseases; osteoporosis; ESTROGEN-RECEPTOR-ALPHA; NITRIC-OXIDE SYNTHASE; CARDIOVASCULAR-DISEASE; OXIDATIVE STRESS; GENE-EXPRESSION; POSTMENOPAUSAL; DENSITY;

D O I：

10.3390/antiox13020241

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Aging and menopause are associated with oxidative stress and inflammation. Here, we evaluated the antioxidant properties of pumpkin (Cucurbita pepo L.) seed extract and assessed its ameliorative effects on aging- and menopause-related diseases using Saos-2 cells and ovariectomized rats. The seed extract had bioactive components that exhibited antioxidant activity. The extract increased the alkaline phosphatase (ALP) activity of Saos-2 cells. The oral administration of the extract to ovariectomized rats for 12 weeks decreased their body weight, fat weight, and cardiac risk indices. It also contributed to reductions in the levels of reactive oxygen species, oxidative stress, and inflammation, as assessed by measuring the serum levels of malondialdehyde and analyzing gene expression in rats. Furthermore, the administration of the extract also promoted an enhancement of the transcription of nuclear factor erythroid 2-related factor (Nrf2), heme oxygenase-1 (Ho-1), and catalase (Cat), involved in antioxidant activity; endothelial nitric oxide synthase (eNos), involved in vasculoprotective activity; and PR/SET domain 16 (Prdm16) and peroxisome proliferator-activated receptor-gamma coactivator (Pgc1 alpha), involved in brown adipogenesis and thermogenesis. Our results using ovariectomized rats show that pumpkin seed extract may have ameliorative effects on menopause-related diseases by increasing ALP activity, evaluating the antioxidant system, ameliorating oxidative stress and thermogenesis, and enhancing lipid profiles.

引用

页数：21