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- [1] Photoinactivation of the bacteriophage PhiX174 by UVA radiation and visible light in SM buffer and DMEM-F12BMC Research Notes, 17Florian SommerfeldLaura WeyersbergPetra VatterMartin Hessling
Photoinactivation of the bacteriophage PhiX174 by UVA radiation and visible light in SM buffer and DMEM-F12
被引：0
|作者：
Sommerfeld, Florian
[1
]
Weyersberg, Laura
[1
]
Vatter, Petra
[1
]
Hessling, Martin
[1
]
机构：
[1] Ulm Univ Appl Sci, Dept Mechatron & Med Engn, Albert Einstein Allee 55, D-89081 Ulm, Germany
关键词：
PhiX174;
UVA;
Visible irradiation;
Radiation inactivation;
Blue light;
Violet light;
External photosensitizers;
Riboflavin;
INACTIVATION;
RIBOFLAVIN;
ULTRAVIOLET;
EXPOSURE;
VIRUSES;
WATER;
DNA;
D O I：
10.1186/s13104-023-06658-8
中图分类号：
Q [生物科学];
学科分类号：
07 ;
0710 ;
09 ;
摘要：
ObjectiveIt has been observed that viruses can be inactivated by UVA radiation and visible light. The aim of this study is to investigate whether a medium that contains a photosensitizer might have an influence on viral reduction under irradiation by UVA, violet or blue light. Test virus is the bacteriophage PhiX174 in the photosensitizer-free SM buffer and DMEM-F12, which contains the known photosensitizer riboflavin.ResultsThe determined PhiX174 D90 doses in SM buffer and DMEM were 36.8 J/cm(2) and 13.6 J/cm(2) at 366 nm, 153.6 J/cm(2) and 129.1 J/cm(2) at 408 nm and 4988 J/cm(2) and 2477.1 J/cm(2) at 455 nm, respectively. It can be concluded that the medium has a large influence on the results. This might be caused by the photosensitizer riboflavin in DMEM-F12. As riboflavin is a key component in many cell culture media, irradiation experiments with viruses in cell culture media should be avoided if the investigation of intrinsical photoinactivation properties of viruses is aimed for.
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