Reversible Fluorescent Banding Analysis in Six Wild Corchorus Species from Bangladesh

Fluorescence chromosome bandings with CMA-and DAPI-were analyzed in six wild Corchorus species to find out the diversity in the number and location of AT-and GC-rich sequences. These six wild Corchorus species had variation in somatic chromosome numbers viz. C. brevicornatus (2n= 14), C. septen-trionalis (2n= 14), C. urticifolius (2n= 14), C. hirtus (2n= 28), C. siliquosus (2n= 28) and C. pinnatipartitus (2n= 78). A wide range of CMA-and DAPI-positive bands were found in the metaphase chromosomes of six wild Corchorus species. A total number of 35 and 32 terminal CMA-and DAPI-bands were generated which recom-mended the tendency of accumulation of GC-and AT-rich sequences at the terminal region of chromosomes, respectively. In some species, CMA-banded satellites were extended in prometaphase while contracted at the terminal region of the short arm in the metaphase stage. Reshuffling of chromosomal segments was detected fol-lowing CMA-and DAPI-banding. After reversible fluorescent banding, C. brevicornatus, C. urticifolius and C. siliquosus had a few CMA-positive and DAPI-negative banded chromosomes which could be used as marker chromosomes. In addition, few chromosomes of C. pinnatipartitus could be used as markers due to their unique DAPI-banding pattern. Each species had its characteristic CMA-and DAPI-banding pattern.