Molecular Cytological Analysis and Specific Marker Development in Wheat-Psathyrostachys huashanica Keng 3Ns Additional Line with Elongated Glume

被引:0
|
作者
Pang, Jingyu [1 ,2 ,3 ]
Huang, Chenxi [1 ,2 ,3 ]
Wang, Yuesheng [1 ,2 ,3 ]
Wen, Xinyu [1 ,2 ,3 ]
Deng, Pingchuan [1 ,2 ,3 ]
Li, Tingdong [1 ,2 ,3 ]
Wang, Changyou [1 ,2 ,3 ]
Liu, Xinlun [1 ,2 ,3 ]
Chen, Chunhuan [1 ,2 ,3 ]
Zhao, Jixin [1 ,2 ,3 ]
Ji, Wanquan [1 ,2 ,3 ]
机构
[1] Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Xianyang 712100, Peoples R China
[2] Northwest A&F Univ, Coll Agron, Xianyang 712100, Peoples R China
[3] Minist Agr, Shaanxi Res Stn Crop Gene Resources & Germplasm En, Xianyang 712100, Peoples R China
关键词
long glume; Psathyrostachys huashanica Keng; molecular cytogenetics; RNA-seq; marker development; TRITICUM-POLONICUM; SUBSTITUTION LINE; GENETIC DIVERSITY; STRIPE RUST; DNA; SEQUENCES; LEYMUS; INHERITANCE; AEGILOPS; GENOME;
D O I
10.3390/ijms24076726
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Psathyrostachys huashanica Keng (2n = 2x = 14, NsNs) is an excellent gene resource for wheat breeding, which is characterized by early maturity, low plant height, and disease resistance. The wheat-P. huashanica derivatives were created by the elite genes of P. huashanica and permeate into common wheat through hybridization. Among them, a long-glume material 20JH1155 was identified, with larger grains and longer spike than its parents. In the present study, the methods of cytological observation, GISH, and sequential FISH analysis showed that 20JH1155 contained 21 pairs of wheat chromosomes and a pair of P. huashanica. There were some differences in 5A and 7B chromosomes between 20JH1155 and parental wheat 7182. Molecular marker, FISH, and sequence cloning indicated 20JH1155 alien chromosomes were 3Ns of P. huashanica. In addition, differentially expressed genes during immature spikelet development of 20JH1155 and 7182 and predicted transcription factors were obtained by transcriptome sequencing. Moreover, a total of 7 makers derived from Ph#3Ns were developed from transcriptome data. Taken together, the wheat-P. huashanica derived line 20JH1155 provides a new horizon on distant hybridization of wheat and accelerates the utilization of genes of P. huashanica.
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页数:15
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