Establishment and Characterization of a Primary Fibroblast Cell Culture from the Amazonian Manatee (Trichechus inunguis)

被引:4
|
作者
Tavares, Flavia dos Santos [1 ]
Martins, Cesar [2 ]
Delella, Flavia Karina [2 ]
do Nascimento, Luis Adriano Santos [3 ]
Rodrigues, Angelica Lucia Figueiredo [4 ]
Moreira, Savia [4 ]
Cardoso, Adauto Lima [1 ,2 ,3 ]
Noronha, Renata Coelho Rodrigues [1 ]
机构
[1] Univ Fed Para, Ctr Estudos Avancados Biodiversidade, Lab Genet & Biol Celular, Inst Ciencias Biol, BR-66075110 Belem, Para, Brazil
[2] Sao Paulo State Univ, Inst Biosci Botucatu, Dept Struct & Funct Biol, UNESP, BR-18618689 Botucatu, SP, Brazil
[3] Univ Fed Para, Lab Oleos Amazonia, BR-66075110 Belem, Para, Brazil
[4] Inst Biol & Conservacao Mamiferos Aquat Amazonia B, BR-66077830 Belem, Para, Brazil
来源
ANIMALS | 2024年 / 14卷 / 05期
基金
巴西圣保罗研究基金会;
关键词
Amazon biobank; cryopreservation; conservation; sirenians; aquatic mammals; endangered species; andiroba; CONSERVATION;
D O I
10.3390/ani14050686
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
The vulnerable status of the Amazon manatee, Trichechus inunguis, indicates the need to seek measures to guarantee its conservation. In this context, the cultivation of cells in vitro is a strategy that should at least guarantee the preservation of their genetic material. Thus, we established for the first time a primary culture of Amazonian manatee fibroblasts (TINsf) from a skin biopsy of a young male. Karyotypic analysis of the 3rd, 7th, and 12th passages confirmed the taxonomic identity of the species T. inunguis (2n = 56/NF = 92) and indicated that this culture presents genomic stability. Gene and protein expression of vimentin at the 13th passage show the predominant presence of fibroblasts in TINsf. To test the cell line's responsiveness to materials and demonstrate a possible application of this culture, it was exposed to andiroba seed oil (ASO), and its viability and proliferative capacity were evaluated. ASO demonstrated toxic effects at the highest concentrations and longest exposure times tested, reproducing results observed in human cultures, indicating the applicability of TINsf in toxicological and biotechnological studies. After cryopreservation, the TINsf line maintained its proliferative potential, indicating the establishment of a new culture available for future studies.
引用
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页数:12
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