Response surface based optimization of laccase production from Perenniporia subtephropora and its application in decolorization of dyes

Laccases from white rot fungi, Perenniporia subtephropora, were collected and studied for the production of the enzyme. In order to increase the efficacy of the enzyme laccase, optimization studies were carried out. Perenniporia subtephropora was optimized by the statistical method Box-Behnken design with four variables (carbon, nitrogen, pH, and temperature) at a time. The higher laccase activity was found to be pH 6.68, temperature 26.49 oC, sucrose content 34.54 g/L, and yeast extract content 4.07 g/L. A mean value of 2423.4 +/- 2.18 (U/L) (N = 5) for laccase acquired from real trials validated the RSM model, indicating that the model was satisfactory. Based on the findings, the crude laccase was tested for decolorization activity against five different textile colors. Direct blue 1 exhibited a maximum decolorization yield of 99%. Further, UV-VIS spectral analysis showed that direct blue 1 was completely degraded by the laccase over a 24-h time period.