Simultaneous Determination of Three Flavonoids in Rat Plasma of Masson Pinus Needles (Pinus massoniana L.) Extracts by UHPLC-MS/MS and Application to Pharmacokinetics Studies after Oral and Transdermal Administration

被引：0
|
作者：
Xia, Chenjie [1 ,2 ]
Zhu, Hongliu [1 ]
Hu, Changhui [2 ]
Liu, Jie [2 ]
Hua, Haibing [1 ]
Yang, Jianqiu [1 ]
Chen, Zhipeng [2 ]
Gao, Yihong [1 ]
机构：
[1] Nanjing Univ Chinese Med, Jiangyin Hosp, Jiangyin 214400, Peoples R China
[2] Nanjing Univ Chinese Med, Coll Pharm, Nanjing 210023, Peoples R China
来源：
JOURNAL OF CHROMATOGRAPHIC SCIENCE | 2024年 / 62卷 / 04期
基金：
中国国家自然科学基金;
关键词：
Pine massoniana needle; alopecia; UHPLC-MS/MS; pharmacokinetics;
D O I：
10.1093/chromsci/bmad074
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Pinusmassoniananeedles, atraditionalherb, wereappliedtopreventhairlossinChina. Studiesavailablemainlyfocusedonpineneedleflavonoidswithvariousbiologicalactivities. However, therehasbeennopharmacokineticsstudyoftheflavonoidsfromPinusneedlesextract. Aselectiveandsensitiveultra-highperformanceliquidchromatography-tandemmassspectrometry(UHPLC-MS/MS) methodwasdevelopedtosimultaneouslyquantifytaxifolin, quercetinandcatechininratplasma. Toseparatethethreeconstituents, anAgilentExtendC18column(2.1mmx100mm, 1.8 mu m) wasusedwithamobilephraseof(A) 0.1% formicacidand(B) acetonitrile. Theanalytesweremeasuredbymultiplereactionmonitoringinthenegativeionizationmode. TherewasgoodlinearityintheestablishedUHPLCMS/MSmethod, withacoefficientofdetermination(r(2)) of>0.99. Theaccuracy, intra-dayandinter-dayprecisionandrecoverywereallsatisfactoryandthese3compoundswerestableunderthetestedconditions. ThevalidatedmethodinthisstudywassuccessfullyappliedtopharmacokineticstudyinhealthyratsafteroralandtransdermaladministrationofPinusneedlesextract.Theresultscouldprovidefurtherresearchfoundationforpineneedleextractasexternalpreparations.
引用
收藏
页码：321 / 327
页数：7
相关论文
共 50 条
  • [1] Simultaneous Determination and Pharmacokinetics Study of Six Triterpenes in Rat Plasma by UHPLC-MS/MS after Oral Administration of Sanguisorba officinalis L. Extract
    Wu, Chengcui
    Yao, Meicun
    Li, Wa
    Cui, Binbin
    Dong, Hongrui
    Ren, Yixuan
    Yang, Chunjuan
    Gan, Chunli
    MOLECULES, 2018, 23 (11):
  • [2] Simultaneous determination and pharmacokinetics studies of five isoflavones in rat plasma by UHPLC-MS/MS after oral administration of Radix Astragali extract
    Han, Jianwei
    Zhu, Wenbo
    Yu, Ling
    Chen, Yajun
    Wu, Gaosong
    Wang, Zhibin
    ACTA CHROMATOGRAPHICA, 2022, 34 (01) : 24 - 31
  • [3] Simultaneous determination of cucurbitacin B and cucurbitacin E in rat plasma by UHPLC-MS/MS: A pharmacokinetics study after oral administration of cucurbitacin tablets
    Wang, Zhibin
    Zhu, Wenbo
    Gao, Mingjie
    Wu, Chengcui
    Yang, Chunjuan
    Yang, Jing
    Wu, Gaosong
    Yang, Bingyou
    Kuang, Haixue
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2017, 1065 : 63 - 69
  • [4] Simultaneous determination of tectorigenin, irigenin and irisflorentin in rat plasma and urine by UHPLC-MS/MS: Application to pharmacokinetics
    Zhang, Wei-dong
    Yang, Wan-jun
    Wang, Xiao-juan
    Gu, Yi
    Wang, Rong
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2011, 879 (31): : 3735 - 3741
  • [5] Simultaneous Determination and Pharmacokinetics of Three Steroidal Alkaloids in Rat Plasma by UHPLC-MS After Oral Administration of Three Different Preparations of Fritillaria ussuriensis Maxim Bulb
    Wu, Haibo
    Yang, Chunjuan
    Chen, Jingnan
    Li, Sen
    Wei, Yuxin
    Wang, Meng
    Li, Xinlan
    Wang, Yanyan
    Kuang, Haixue
    Wang, Zhibin
    BIOMEDICAL CHROMATOGRAPHY, 2025, 39 (03)
  • [6] Simultaneous Determination and Pharmacokinetics Study of Three Triterpenes from Sanguisorba officinalis L. in Rats by UHPLC-MS/MS
    Wei, Fanshu
    Yang, Chunjuan
    Wu, Lihong
    Sun, Jiahui
    Wang, Zhenyue
    Wang, Zhibin
    MOLECULES, 2022, 27 (17):
  • [7] Development and validation of a UHPLC-MS/MS method for the simultaneous determination of five bioactive flavonoids in rat plasma and comparative pharmacokinetic study after oral administration of Xiaochaihu Tang and three compatibilities
    Bo, Yunhai
    Wang, Lijuan
    Wu, Xiao
    Zhao, Longshan
    Yang, Jingyu
    Xiong, Zhili
    Wu, Chunfu
    JOURNAL OF SEPARATION SCIENCE, 2017, 40 (09) : 1896 - 1905
  • [8] An UHPLC-MS/MS method for simultaneous quantification of gallic acid and protocatechuic acid in rat plasma after oral administration of Polygonum capitatum extract and its application to pharmacokinetics
    Ma, Fengwei
    Gong, Xiaojian
    Zhou, Xin
    Zhao, Yang
    Li, Menglin
    JOURNAL OF ETHNOPHARMACOLOGY, 2015, 162 : 377 - 383
  • [9] Simultaneous determination and pharmacokinetic study of eight components in rat plasma by UHPLC-MS/MS after oral administration of Hypericum japonicum Thunb extract
    Pang, Qian
    Tian, Yuanyuan
    Mi, Jianping
    Wang, Jin
    Xu, Yuanjin
    JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2016, 118 : 228 - 234
  • [10] Use of an UHPLC-MS/MS Method for Determination of Kuraridin and Characterization of Its Metabolites in Rat Plasma after Oral Administration
    Liu, Yi
    Chen, Lei
    Cai, Wei
    Zhao, Lin-lin
    Mo, Zhi-xian
    MOLECULES, 2018, 23 (02):
12345