Biosynthesis of Cry5B-Loaded Sulfur Nanoparticles using Arthrobotrys oligospora Filtrate: Effects on Nematicidal Activity, Thermal Stability, and Pathogenicity against Caenorhabditis elegans

被引：1

作者：

Jammor, Pasin ^{[1
]}

Sanguanphun, Tanatcha ^{[2
]}

Meemon, Krai ^{[2
]}

Promdonkoy, Boonhiang ^{[3
]}

Boonserm, Panadda ^{[1
]}

机构：

[1] Mahidol Univ, Inst Mol Biosci, Phuttamonthon 73170, Nakhon Pathom, Thailand

[2] Mahidol Univ, Fac Sci, Dept Anat, Bangkok 10400, Thailand

[3] Natl Sci & Technol Dev Agcy, Natl Ctr Genet Engn & Biotechnol, Khlong Luang 12120, Pathumthani, Thailand

来源：

ACS OMEGA | 2024年 / 9卷 / 06期

关键词：

ANTIBACTERIAL; NEMATODES; DELIVERY; BEHAVIOR; SILICA; FUNGI; CELLS;

D O I：

10.1021/acsomega.3c08653

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Cry5B, a crystal protein produced by Bacillus thuringiensis (Bt), is a bionematicide with potent nematicidal activity against various plant-parasitic and free-living nematodes. This protein, however, is susceptible to destruction by ultraviolet light, proteolytic enzymes, and high temperatures. This study aims to produce Cry5B protein for bionematicidal use and improve its stability and nematicidal efficacy by loading it intoArthrobotrys oligospora-mediated sulfur nanoparticles (AO-SNPs). Based on the mortality assay, the Cry5B protein exhibited dose-dependent nematicidal activity against the model organismCaenorhabditis elegans. The nematicidal activity, thermal stability, and pathogenic effects of Cry5B-loaded AO-SNPs (Cry5B-SNPs) were compared to those of free Cry5B. After 3 h of exposure to heat at 60 C-degrees, Cry5B-SNPs had greater nematicidal activity than free Cry5B protein, indicating the effective formulation of Cry5B-SNPs that could be used as an alternative to current nematicide delivery strategies.

引用

页码：6945 / 6954

页数：10