Obtaining and characterization of spheroids using colon adenocarcinoma SW480 cells

被引：0
|
作者：
Ceballos-Duque, Susana [1 ]
Maldonado-Celis, Maria Elena [1 ]
Montoya, Yuliet [2 ]
机构：
[1] Univ Antioquia, Escuela Nutr & Dietet, Medellin, Colombia
[2] Univ Pontificia Bolivariana, Ctr Bioingn, Medellin, Colombia
来源：
REVISTA COLOMBIANA DE CANCEROLOGIA | 2023年 / 27卷 / 03期
关键词：
Spheroids; cellular; colonic neoplasms; cell death; cell cycle; mem-brane potential; mitochondrial; CULTURE; 3D;
D O I：
10.35509/01239015.957
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Colon cancer has conventionally been studied using in vitro monolayer models to understand its biology and identify therapeutic agents; nevertheless, they do not represent tumor cells growing in three dimensions. This technical note presents a protocol to develop and characterize a three-dimensional model of spheroids of different sizes, formed with SW480 colon adenocarcinoma cells seeded on agarose micromolds allowed to grow for up to 21 days. The spheroids were characterized by flow cytometry based on cell cycle, mitochondrial function, and apoptosis. Spheroids of 400 mu m seeding size and 3 days of growth were found to represent a model possessing similar characteristics to those of tumors in vivo. An easy-to -implement method to evaluate therapeutic agents is proposed, previous to the use of animal models.
引用
收藏
页码：389 / 396
页数：8
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