Myh6 promoter-driven Cre recombinase excises floxed DNA fragments in a subset of male germline cells

被引：1

作者：

Sheldon, Caroline ^{[1
]}

Kessinger, Chase W. ^{[1
]}

Sun, Yan ^{[1
]}

Kontaridis, Maria I. ^{[1
,2
,3
]}

Ma, Qianyi ^{[4
]}

Hammoud, Saher Sue ^{[4
,5
,6
,7
]}

Gao, Zibei ^{[8
]}

Zhang, Hui ^{[8
]}

Lin, Zhiqiang ^{[1
]}

机构：

[1] Masonic Med Res Inst, 2150 Bleecker St, Utica, NY 13501 USA

[2] Beth Israel Deaconess Med Ctr, Dept Med, Div Cardiol, Boston, MA 02115 USA

[3] Harvard Med Sch, Dept Biol Chem & Mol Pharmacol, Boston, MA USA

[4] Univ Michigan, Dept Human Genet, Ann Arbor, MI USA

[5] Univ Michigan, Dept Obstet & Gynecol, Ann Arbor, MI USA

[6] Univ Michigan, Cellular & Mol Biol Program, Ann Arbor, MI USA

[7] Univ Michigan, Dept Urol, Ann Arbor, MI USA

[8] ShanghaiTech Univ, Sch Life Sci & Technol, 393 Middle Huaxia Rd, Shanghai 201210, Peoples R China

来源：

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY | 2023年 / 175卷

关键词：

Myh6; promoter; Cre; tdTom reporter; Germline cells; DELETION; ACTIVATION;

D O I：

10.1016/j.yjmcc.2022.12.005

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Myh6-Cre transgenic mouse line was known to express Cre recombinase only in the heart. Nevertheless, during breeding Myh6-Cre to Rosa26fstdTom reporter (tdTom) mouse line, we observed that a significant part of their F2 tdTom/+ offspring had tdTom reporter gene universally activated. Our results show that Myh6-Cre transgenic mice have Cre recombinase activity in a subpopulation of the male germline cells, and that Myh6 gene transcripts are enriched in the interstitial Leydig cells and the undifferentiated spermatogonia stem cells. In summary, the current study confirms that the previously known "heart-specific" Myh6 promoter drives Cre expression in the testis.

引用

页码：62 / 66

页数：5