This study explores the extraction and application of a sustainable dye derived from Talaromyces purpurogenus fungi for dyeing silk fabric. The fungal dye imparted a vibrant red shade to silk, offering a promising alternative to synthetic dyes. The dyeing process was optimized using the Box and Behnken Design of RSM software, focusing on dye pH, dyeing temperature, and dyeing time. Silk fabric was dyed using an HTHP dyeing machine, and its performance was assessed in terms of colour strength, percent absorption, light fastness, rubbing fastness, and washing fast-ness. SEM imaging and FT-IR spectroscopy were employed to investigate the interaction between the dye and silk fabric. The optimized dyeing process resulted in silk fabric with a satisfactory red shade, demonstrating favorable fastness ratings at pH 5. The optimal dyeing conditions included a dye pH of 5, dyeing temperature of 82 degrees C, and dyeing time of 55 minutes, with an optical density of 10 and MLR of 1:30. Silk fabric exhibited maximum dye absorption capacity at pH 5, while maintaining excellent washing and rubbing fastness properties. However, the light fastness of the dyed silk fabric was relatively poor. Microscopic examination revealed a rough surface texture. Overall, this study highlights the potential of T. purpurogenus fungal dye as a sustainable alter-native for silk fabric dyeing, while emphasizing the importance of addressing challenges such as improving light fastness through surface treatment and further exploring dyeing techniques.