A fluorescence biosensor based on DNA aptamers-COF for highly selective detection of ATP and thrombin

被引：12
|
作者：
Jouha, Jabrane [1 ]
Li, Fengli [1 ]
Xiong, Hai [1 ]
机构：
[1] Shenzhen Univ, Inst Adv Study, Shenzhen 518060, Peoples R China
来源：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2023年 / 295卷
关键词：
2D-COF; Biosensing; G4-forming aptamer; ATP; Thrombin detection; COVALENT ORGANIC FRAMEWORKS; ENERGY-STORAGE; CRYSTALLINE; NANOSHEETS; IMINE; 2D; PERFORMANCE; DESIGN;
D O I：
10.1016/j.saa.2023.122615
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
Due to their distinctive physical, chemical, electrical, and optical properties as well as their prospective uses, 2D covalent organic framework (COF) have attracted much attention. Herein, TaTPA-COF was effectively synthesized from the condensation of TTA and TFPA using a facile solvothermal method and characterized by SEM image, FT-IR spectra, and PXRD pattern. The generated bulk TaTPA-COF materials combined with DNA aptamers are utilized as the acceptor (quencher) for the highly sensitive and selective detection of adenosine 5 '-triphosphate (ATP) and thrombin, with a novel fluorescence biosensing platform and a proof-of-concept application.
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页数：5
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