A novel Mutidrug and Toxic Compound Extrusion (MATE) gene SmMATE1 from Salvia miltiorrhiza Bunge, is involved in tetracycline induced mitochondrial toxicity adaptation synergically with salvianolic acid B

被引：2

作者：

Zhong, Mingzhi ^{[1
,2
]}

Yu, Haomiao ^{[1
,2
]}

Jiang, Yuanyuan ^{[1
,2
]}

Liao, Jinqiu ^{[1
,4
]}

Chai, Songyue ^{[1
,2
]}

Wang, Long ^{[1
,3
]}

Yang, Ruiwu ^{[1
,4
]}

Zhou, Yonghong ^{[1
,3
]}

Wang, Yi ^{[1
,3
]}

Zeng, Jian ^{[1
,5
]}

Kang, Houyang ^{[3
]}

Zhang, Li ^{[1
,2
]}

机构：

[1] Sichuan Agr Univ, Featured Med Plants Sharing & Serv Platform, Yaan 625014, Peoples R China

[2] Sichuan Agr Univ, Coll Sci, Yaan 625014, Peoples R China

[3] Sichuan Agr Univ, Triticeae Res Inst, Chengdu 611130, Peoples R China

[4] Sichuan Agr Univ, Coll Life Sci, Yaan 625014, Peoples R China

[5] Sichuan Agr Univ, Coll Resources, Chengdu 611130, Peoples R China

来源：

INDUSTRIAL CROPS AND PRODUCTS | 2024年 / 209卷

关键词：

MATE transporter; Salvianolic acid B; Molecular evolution; Yeast expression; Hairy root; HUMAN MULTIDRUG; STRESS; TRANSPORTERS; ANTIBIOTICS; PLANTS; GROWTH; INHIBITION; SELECTION; PROTEINS; ACCURACY;

D O I：

10.1016/j.indcrop.2023.117939

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

As a tetracycline antibiotic, the enrichment of doxycycline in soil seriously endangers agricultural cultivation and food security. Salvianolic acid B (SAB), as a natural product, has high antioxidant activity. We applied SAB and doxycycline (DOX) externally to Salvia miltiorrhiza seedlings, and measured the activities of total superoxide dismutase, peroxidase and catalase, as well as the accumulation of malondialdehyde, active oxygen and glutathione. It was found that SAB can participate in the DOX stress release of S. miltiorrhiza through the antioxidant system. We also analyzed the evolutionary relationship of multidrug and toxic compound extrusion (MATE) family proteins related to antibiotic transport in 50 representative evolutionary genomes, and revealed the potential binding ability of MATE and DOX through macromolecular docking. Finally, based on transcriptome and RT-qPCR analysis, we identified a novel gene SmMATE1, and found that it has a co-expression pattern with SmRAS and SmCYP98A14. Transgenic in tobacco, yeast and hairy root of S. miltiorrhiza revealed the important role of this new gene and SAB in synergistically releasing DOX stress damage, which provided a reference for natural product to alleviate the stress of antibiotics on plants, and also provided a theoretical basis for the development of new soil antibiotic sustained-release agents.

引用

页数：18