Artemisinin promotes apoptosis of spinal tuberculosis macrophages by inhibiting NF-KB

Artemisinin has been proved to have a wide range of pharmacological effects, mainly used in anti -malaria, anti-inflammatory, antiviral, etc., but the effect and mechanism of artemisinin on tuberculosis-infected macrophages have not yet been elucidated. THP-1 cell line was cultured and randomly divided into the following 4 groups; control group, which was infected with Mtb standard human virulence strain H37Rv; low-dose, medium-dose and high-dose artemisinin groups, which were treated with artemisinin at 50 mu M, 150 mu M and 300 mu M, respectively. The effect of artemisinin on cell proliferation was determined by MTT assay. MMP-2 and MMP-9 were detected by PCR. The inflammatory cytokines IL-6 and TNF-a were detected by ELISA. The above tests were used to understand apoptosis,inflammation and proliferation. The expressions IP: 217.163118.10 On: Tue, 18 Apr 2023 14 07:53 of Bcl-2, osteoprotectin (OPG), nucleafactor KB receptor activator (RANK) and NF-KB were detected by Copyr ight: Amer can Scientific Publ shers Western blot. Acting on macrophages infected with mycobacterium tuberculosis, artemisinin can significantly Delivered by Ingenta inhibit cell proliferation, promote cell apoptosis, inhibit the expression of MMP-2 and MMP-9, and reduce the secretion of IL-6 and TNF-a. Artemisinin also decreased the expression of Bcl-2, OPG, RANK and NF-KB (p < 0.05), and the change was more significant with the increase of dose. Artemisinin could down-regulate the expression of OPG, RANK and NF-KB, and inhibiting the expression of inflammatory factors and Matrix metalloproteinases (MMPs), and then promoting apoptosis of spinal tuberculosis macrophages.