PrimeRoot for targeted large DNA insertion in plants

被引：1
|
作者：
Pan, Changtian [1 ,2 ]
Qi, Yiping [2 ,3 ]
机构：
[1] Zhejiang Univ, Coll Agr & Biotechnol, Dept Hort, Hangzhou, Peoples R China
[2] Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20742 USA
[3] Univ Maryland, Inst Biosci & Biotechnol Res, Rockville, MD 20850 USA
来源：
TRENDS IN PLANT SCIENCE | 2023年 / 28卷 / 08期
基金：
中国国家自然科学基金;
关键词：
D O I：
10.1016/j.tplants.2023.05.002
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Genome editing technologies such as clustered regularly interspaced short palindromic repeats (CRISPR) have revolutionized plant breeding through targeted genome and transcriptome modifications. However, accurate insertion of large DNA cargoes remains challenging. Recently, Sun and colleagues introduced PrimeRoot, a groundbreaking technology that enables precise and targeted integration of large DNA cargoes into plant genomes with remarkable efficiency and accuracy.
引用
收藏
页码：870 / 872
页数：3
相关论文
共 50 条
  • [1] Targeted DNA insertion in plants
    Dong, Oliver Xiaoou
    Ronald, Pamela C.
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2021, 118 (22)
  • [2] Efficient targeted insertion of large DNA fragments without DNA donors
    Wang, Jinlin
    He, Zhou
    Wang, Guoquan
    Zhang, Ruiwen
    Duan, Junyi
    Gao, Pan
    Lei, Xinlin
    Qiu, Houyuan
    Zhang, Chuanping
    Zhang, Ying
    Yin, Hao
    NATURE METHODS, 2022, 19 (03) : 331 - +
  • [3] Efficient targeted insertion of large DNA fragments without DNA donors
    Jinlin Wang
    Zhou He
    Guoquan Wang
    Ruiwen Zhang
    Junyi Duan
    Pan Gao
    Xinlin Lei
    Houyuan Qiu
    Chuanping Zhang
    Ying Zhang
    Hao Yin
    Nature Methods, 2022, 19 : 331 - 340
  • [4] Precise integration of large DNA sequences in plant genomes using PrimeRoot editors
    Sun, Chao
    Lei, Yuan
    Li, Boshu
    Gao, Qiang
    Li, Yunjia
    Cao, Wen
    Yang, Chao
    Li, Hongchao
    Wang, Zhiwei
    Li, Yan
    Wang, Yanpeng
    Liu, Jun
    Zhao, Kevin Tianmeng
    Gao, Caixia
    NATURE BIOTECHNOLOGY, 2024, 42 (02) : 316 - 327
  • [5] Precise integration of large DNA sequences in plant genomes using PrimeRoot editors
    Chao Sun
    Yuan Lei
    Boshu Li
    Qiang Gao
    Yunjia Li
    Wen Cao
    Chao Yang
    Hongchao Li
    Zhiwei Wang
    Yan Li
    Yanpeng Wang
    Jun Liu
    Kevin Tianmeng Zhao
    Caixia Gao
    Nature Biotechnology, 2024, 42 : 316 - 327
  • [6] Beyond a few bases: methods for large DNA insertion and gene targeting in plants
    Vollen, Katie
    Alonso, Jose M.
    Stepanova, Anna N.
    PLANT JOURNAL, 2025, 121 (06):
  • [7] A possible aid in targeted insertion of large DNA elements by CRISPR/Cas in mouse zygotes
    Nakao, Harumi
    Harada, Takeshi
    Nakao, Kazuki
    Kiyonari, Hiroshi
    Inoue, Kenichi
    Furuta, Yasuhide
    Aiba, Atsu
    GENESIS, 2016, 54 (02) : 65 - 77
  • [8] Transcription Activator-Like Effector Nucleases (TALEN)-Mediated Targeted DNA Insertion in Potato Plants
    Forsyth, Adrienne
    Weeks, Troy
    Richael, Craig
    Duan, Hui
    FRONTIERS IN PLANT SCIENCE, 2016, 7
  • [9] P[acman]:: A BAC transgenic platform for targeted insertion of large DNA fragments in D-melanogaster
    Venken, Koen J. T.
    He, Yuchun
    Hoskins, Roger A.
    Bellen, Hugo J.
    SCIENCE, 2006, 314 (5806) : 1747 - 1751
  • [10] Targeted DNA cleavage and insertion by group II introns
    Lambowitz, A
    Guo, H
    Zimmerly, S
    Mohr, G
    Perlman, P
    FASEB JOURNAL, 1997, 11 (09): : A1446 - A1446
12345