Detection of Clostridium botulinum in Some Egyptian Fish Products, Its Control In Vitro Using Citrus Leaves Extracts, and Applicability of Citrus limon Leaf Extract in Tuna

被引：2

作者：

Hamad, Gamal ^{[1
]}

Hafez, Elsayed E. ^{[2
]}

Sobhy, Sherien E. ^{[2
]}

Mehany, Taha ^{[1
]}

Elfayoumy, Reham A. ^{[3
]}

Elghazaly, Eman M. ^{[4
]}

Eskander, Michael ^{[5
]}

Tawfik, Rasha G. ^{[6
]}

Hussein, Saleh M. ^{[7
]}

Pereira, Leonel ^{[8
]}

机构：

[1] Arid Lands Cultivat Res Inst, Dept Food Technol, City Sci Res & Technol Applicat, New Borg El Arab 21934, Egypt

[2] Arid Lands Cultivat Res Inst, Dept Plant Protect & Biomol Diag, City Sci Res & Technol Applicat, New Borg El Arab 21934, Egypt

[3] Damietta Univ, Fac Sci, Dept Bot & Microbiol, Dumyat 34511, Egypt

[4] Matrouh Univ, Fac Vet Med, Dept Microbiol, Matrouh 51511, Egypt

[5] Alexandria Univ, Fac Vet Med, Dept Food Hyg & Control, Alexandria 22758, Egypt

[6] Alexandria Univ, Fac Vet Med, Dept Microbiol, Alexandria 22758, Egypt

[7] Al Azhar Univ, Fac Agr, Dept Food Sci & Technol, Assiut 71524, Egypt

[8] Univ Coimbra, MARE Marine & Environm Sci Ctr, Dept Life Sci, P-3000456 Coimbra, Portugal

来源：

FOODS | 2023年 / 12卷 / 07期

关键词：

Clostridium botulinum; natural antioxidants; multiplex PCR; fishery products; Citrus; natural antimicrobials; natural extracts; shelf-life; botulism sickness; IDENTIFICATION; ANTIOXIDANT; QUALITY; PEEL;

D O I：

10.3390/foods12071466

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

This study aims to detect Clostridium botulinum and its control using natural leaf extracts of Citrus limon, Citrus sinensis, and Citrus unshiu in Egyptian fish products, e.g., canned tuna, canned sardine, canned mackerel, fesikh, moloha, and renga, as well the application of C. limon in tuna. Moreover, the antibacterial activity of the C. limon leaf extract was also estimated. In the water extract, ascorbic acid, total flavonoid content (TFC), and total phenolic content (TPC) were determined by volumetric, aluminum chloride, and Folin-Ciocalteu approaches, respectively. The antioxidant ability of the extract was analyzed in vitro via free radical scavenging (DPPH) and Ferric reducing assays. The results showed variability in the distribution of the total number of positive C. botulinum in fish samples from three different governorates under study, which were (24) Alexandria, (16) Beheira, and (17) Gharbia, out of the 120 tested samples in each governorate. Additionally, the findings revealed that all three Citrus extracts contain an appropriate number of secondary metabolites, with a sustainable presence of saponin and tannins in the C. limon extract. Furthermore, all Citrus extracts inhibited bacterial growth by increasing the inhibition zone, with C. limon being the best extract (25 mm) compared to C. sinensis and C. unshiu. The overall results showed the high antioxidant and anti-Clostridium powers (p < 0.05) of C. limon leaf extract, indicating its preservative activity in fishery products during storage. Finally, C. limon leaf extract can fight off C. botulinum and is considered a promising natural preservation candidate in ensuring safe and fresh fishery products.

引用

页数：15