Transient gene expression in petunia flower petals as a toolbox

被引：0
|
作者：
Duan, Hui [1 ,2 ]
机构：
[1] USDA ARS, Floral & Nursery Plants Res Unit, US Natl Arboretum, Beltsville, MD USA
[2] USDA ARS, Floral & Nursery Plants Res Unit, US Natl Arboretum, 10300 Baltimore Ave Bldg 010A, Beltsville, MD 20705 USA
来源：
JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY | 2024年 / 99卷 / 02期
基金：
美国农业部;
关键词：
Agro-infiltration; Petunia x hybrida; flower petal; transient expression; gene editing; TOMATO FRUIT; RAPID ASSAY; AGROINFILTRATION; RESISTANCE;
D O I：
10.1080/14620316.2023.2248127
中图分类号：
S6 [园艺];
学科分类号：
0902 ;
摘要：
Agroinfiltration-mediated transient expression is widely used in plant gene functional studies. In this study, the functionality of a metabolic engineering construct containing multiple expression cassettes for betalain biosynthesis genes was validated via agroinfiltration in petunia flower petals. The effects of transcription factors that upregulate anthocyanin biosynthesis and a gene that regulates cellular pH were also demonstrated on petunia petals by agroinfiltration. We also showed that flower petal agroinfiltration is a quick approach to test gene editing components, e.g. different guide RNAs. In general, the agroinfiltration-mediated transient transformation in flower petals is a great tool for testing flowering colour-related trait genes, as well as for optimising gene editing components.
引用
收藏
页码：160 / 167
页数：8
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