ASV portal: an interface to DNA-based biodiversity data in the Living Atlas

被引:2
|
作者
Prager, Maria [1 ,2 ]
Lundin, Daniel [3 ]
Ronquist, Fredrik [4 ]
Andersson, Anders F. [5 ]
机构
[1] Stockholm Univ, Dept Ecol Environm & Plant Sci, Sci Life Lab, S-10691 Stockholm, Sweden
[2] Karolinska Inst, Dept Microbiol Tumor & Cell Biol, S-17177 Stockholm, Sweden
[3] Linnaeus Univ, Ctr Ecol & Evolut Microbial Model Syst, S-39182 Kalmar, Sweden
[4] Swedish Museum Nat Hist, Dept Bioinformat & Genet, POB 50007, S-10405 Stockholm, Sweden
[5] KTH Royal Inst Technol, Dept Gene Technol, Sci Life Lab, S-17121 Stockholm, Sweden
基金
瑞典研究理事会;
关键词
Biodiversity informatics; Species occurrence; Darwin core; Amplicon sequencing; Metabarcoding; eDNA; BLAST;
D O I
10.1186/s12859-022-05120-z
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Background: The Living Atlas is an open source platform used to collect, visualise and analyse biodiversity data from multiple sources, and serves as the national biodiversity data hub in many countries. Although powerful, the Living Atlas has had limited func-tionality for species occurrence data derived from DNA sequences. As a step toward integrating this fast-growing data source into the platform, we developed the Ampli-con Sequence Variant (ASV) portal: a web interface to sequence-based biodiversity observations in the Living Atlas.Results: The ASV portal allows data providers to submit denoised metabarcoding output to the Living Atlas platform via an intermediary ASV database. It also enables users to search for existing ASVs and associated Living Atlas records using the Basic Local Alignment Search Tool, or via filters on taxonomy and sequencing details. The ASV portal is a Python-Flask/jQuery web interface, implemented as a multi-container docker service, and is an integral part of the Swedish Biodiversity Data Infrastructure. Conclusion: The ASV portal is a web interface that effectively integrates biodiversity data derived from DNA sequences into the Living Atlas platform.
引用
收藏
页数:6
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