Leaf Color Classification and Expression Analysis of Photosynthesis-Related Genes in Inbred Lines of Chinese Cabbage Displaying Minor Variations in dark-green Leaves

被引：4

作者：

Su, Xiangjie ^{[1
]}

Yue, Xiaonan ^{[1
]}

Kong, Mingyu ^{[1
]}

Xie, Ziwei ^{[1
]}

Yan, Jinghui ^{[1
]}

Ma, Wei ^{[1
]}

Wang, Yanhua ^{[1
]}

Zhao, Jianjun ^{[1
]}

Zhang, Xiaomeng ^{[1
]}

Liu, Mengyang ^{[1
]}

机构：

[1] Hebei Agr Univ, Coll Hort, Collaborat Innovat Ctr Vegetable Ind Hebei, State Key Lab North China Crop Improvement & Regul, Baoding 071000, Peoples R China

来源：

PLANTS-BASEL | 2023年 / 12卷 / 11期

基金：

中国国家自然科学基金;

关键词：

slight differences in leaf color; leaf color classification; photosynthesis-related genes; relative expression analysis; LIGHT-HARVESTING COMPLEX; PHOTOSYSTEM-II; GRANA MEMBRANES; CHLOROPHYLL-A; ARABIDOPSIS; PROTEINS; FERROCHELATASE; OXYGENASE; ORGANIZATION; SUBUNITS;

D O I：

10.3390/plants12112124

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The leaves of the Chinese cabbage which is most widely consumed come in a wide variety of colors. Leaves that are dark green can promote photosynthesis, effectively improving crop yield, and therefore hold important application and cultivation value. In this study, we selected nine inbred lines of Chinese cabbage displaying slight differences in leaf color, and graded the leaf color using the reflectance spectra. We clarified the differences in gene sequences and the protein structure of ferrochelatase 2 (BrFC2) among the nine inbred lines, and used qRT-PCR to analyze the expression differences of photosynthesis-related genes in inbred lines with minor variations in dark-green leaves. We found expression differences among the inbred lines of Chinese cabbage in photosynthesis-related genes involved in the porphyrin and chlorophyll metabolism, as well as in photosynthesis and photosynthesis-antenna protein pathway. Chlorophyll b content was significantly positively correlated with the expression of PsbQ, LHCA1_1 and LHCB6_1, while chlorophyll a content was significantly negatively correlated with the expression PsbQ, LHCA1_1 and LHCA1_2. Our results provide an empirical basis for the precise identification of candidate genes and a better understanding of the molecular mechanisms responsible for the production of dark-green leaves in Chinese cabbage.

引用

页数：13