A novel way to evaluate autoantibody interference in samples with mixed antinuclear antibody patterns in the HEp-2 cell based indirect immunofluorescence assay and comparison of conventional microscopic and computer-aided pattern recognition

被引：0

作者：

Nagy, Gabor ^{[1
,7
]}

Foldesi, Roza ^{[1
]}

Csipo, Istvan ^{[1
]}

Tarr, Tuende ^{[2
]}

Szucs, Gabriella ^{[3
]}

Szanto, Antonia ^{[2
]}

Buban, Tamas ^{[4
]}

Szekanecz, Zoltan ^{[3
]}

Papp, Maria ^{[4
,5
]}

Kappelmayer, Janos ^{[1
]}

Antal-Szalmas, Peter ^{[1
,6
]}

机构：

[1] Univ Debrecen, Fac Med, Dept Lab Med, Debrecen, Hungary

[2] Univ Debrecen, Fac Med, Dept Clin Immunol, Inst Internal Med, Debrecen, Hungary

[3] Univ Debrecen, Fac Med, Dept Rheumatol, Inst Internal Med, Debrecen, Hungary

[4] Univ Debrecen, Fac Med, Dept Gastroenterol, Debrecen, Hungary

[5] European Reference Network Hepatol Dis, ERN RAREL, LIVER, Debrecen, Hungary

[6] Univ Debrecen, Fac Med, Dept Lab Med, Nagyerdei Str 98, H-4032 Debrecen, Hungary

[7] Univ Debrecen, Gyula Petranyi Doctoral Sch Allergy & Clin Immunol, Debrecen, Hungary

来源：

CLINICA CHIMICA ACTA | 2024年 / 553卷

关键词：

ANA HEp-2 test; Indirect immunofluorescence; Mixed patterns; Multiple patterns; Automated pattern recognition; INTERNATIONAL CONSENSUS;

D O I：

10.1016/j.cca.2023.117747

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Background: A major challenge of the HEp-2 cell-based indirect immunofluorescence (IIF) assays is the correct identification of the individual anti-nuclear antibodies (ANAs) if more than one is present in a sample. We created artificial mixes by pooling two different samples with a single autoantibody in different titers. Comparison of the expected and observed patterns and titers clarifies the interference between the two tested ANAs. Methods: Serum samples with a single homogeneous or speckled ANA pattern were serially diluted and mixed in 16 combinations, providing end-point titers of 1:5,120 to 1:80 for both patterns. These mixes were tested by a HEp-2 IIF assay and were evaluated by conventional evaluation, the EUROPattern (EPa) system and on-screen analysis. Results: Homogeneous pattern can alter the identification of the speckled pattern much more than vice versa, but both has an interfering effect on the other. The effect of the interfering on the tested pattern was higher if the titer of the former one was higher. The pattern recognition efficacy of conventional and the on-screen evaluation was similar and superior compared to the EPa analysis. Conclusions: The application of artificial mixed samples can help the evaluation of the efficacy of manual and computer-aided ANA HEp-2 pattern recognition.

引用

页数：9

共 1 条

[1] The antinuclear antibody HEp-2 indirect immunofluorescence assay: a survey of laboratory performance, pattern recognition and interpretation
Tebo, Anne E.
Schmidt, Robert L.
Kadkhoda, Kamran
Peterson, Lisa K.
Chan, Edward K. L.
Fritzler, Marvin J.
Wener, Mark H.
AUTOIMMUNITY HIGHLIGHTS, 2021, 12 (01)

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