Antiproliferative Activity of New Pyrazole-4-sulfonamide Derivatives: Synthesis and Biological Evaluation

Pyrazole and sulfonamideconstitute an important class of drugs,with several types of pharmacological agents. Facile synthesis oftwo new series of 3,5-dimethyl-1H-pyrazole-4-sulfonamideand 1,3,5-trimethyl-1H-pyrazole-4-sulfonamide derivativeswas designed and synthesized. These pyrazole-4-sulfonamide derivativesare characterized by Fourier transform infrared (FT-IR), H-1 NMR, C-13 NMR, and elemental analysis, and their biologicalevolution data are presented. This paved way for the development ofnew pyrazole-4-sulfonamide derivatives. These compounds are testedfor their in vitro antiproliferative activity against U937 cells bythe CellTiter-Glo Luminescent cell viability assay using MitomycinC. Cytotoxicity detection is based on the measurement of LDH activity,while these compounds did not exhibit cytotoxic activity on thesecells. Half maximal inhibitory concentration (IC50) wascalculated by Graph Pad Prism software for each dose. Their structure-activityrelationships were obtained and discussed.