Synergistic interaction of polymyxin B with carvacrol: antimicrobial strategy against polymyxin-resistant Klebsiella pneumoniae

被引：4
|
作者：
de Souza, Gleyce H. A. [1 ]
Vaz, Marcia S. M. [1 ]
Radai, Joyce A. dos Santos [1 ]
Fraga, Thiago L. [2 ]
Rossato, Luana [1 ]
Simionatto, Simone [1 ]
机构：
[1] Univ Fed Grande Dourados UFGD, Lab Pesquisa Ciencias Saude, Dourados, MS, Brazil
[2] Ctr Univ Grande Dourados UNIGRAN, BR-79824900 Dourados, MS, Brazil
来源：
FUTURE MICROBIOLOGY | 2024年 / 19卷 / 03期
关键词：
Gram-negative; monoterpene; multidrug resistant; natural product; synergy effect; SUSCEPTIBILITY; COMBINATIONS; MECHANISMS; PLASMIDS;
D O I：
10.2217/fmb-2023-0070
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Objective: The antimicrobial activities of the synergistic combination of carvacrol and polymyxin B against polymyxin-resistant Klebsiella pneumoniae were evaluated. Methods: The methods employed checkerboard assays to investigate synergism, biofilm inhibition assessment and membrane integrity assay. In addition, the study included in vivo evaluation using a mouse infection model. Results: The checkerboard method evaluated 48 combinations, with 23 indicating synergistic action. Among these, carvacrol 10 mg/kg plus polymyxin B 2 mg/kg exhibited in vivo antimicrobial activity in a mouse model of infection, resulting in increased survival and a significant decrease in bacterial load in the blood. Conclusion: Polymyxin in synergy with carvacrol represents a promising alternative to be explored in the development of new antimicrobials.
引用
收藏
页码：181 / 193
页数：13
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