Cellular Immune Responses in Lymphoid Tissues of Broiler Chickens Experimentally Infected with Necrotic Enteritis-Producing Clostridium perfringens Strains

被引:1
|
作者
Kulkarni, Raveendra R. [1 ]
Gaghan, Carissa [1 ]
Mohammed, Javid [1 ,2 ]
Sharif, Shayan [3 ]
Taha-Abdelaziz, Khaled [4 ]
机构
[1] North Carolina State Univ, Dept Populat Hlth & Pathobiol, Coll Vet Med, Raleigh, NC 27607 USA
[2] Duke Univ Sch Med, Dept Immunol, Durham, NC 27710 USA
[3] Univ Guelph, Dept Pathobiol, Ontario Vet Coll, Guelph, ON N1G2W1, Canada
[4] Clemson Univ, Dept Anim & Vet Sci, Clemson, SC 29634 USA
关键词
necrotic enteritis; Clostridium perfringens; chickens; immune response; virulence; T-CELLS; IMMUNIZATION; PATHOGENESIS; PREVENTION; VIRULENT; DISEASE;
D O I
暂无
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Host cellular responses against Clostridium perfringens (CP), the causative agent of necrotic enteritis (NE) in chickens, are poorly understood. In the present study, we first tested the NE-producing ability of seven netB(+) CP strains (CP5, CP18, CP26, CP64, CP67, CP68, and NCNE-1), using an experimental infection model of broiler chickens. Evaluation of intestinal gross lesions showed that all the strains, except CP5, were able to produce NE, while CP26 and CP64 strains produced relatively more severe lesions when compared with other groups. Next, cellular responses in the cecal tonsil (CT), bursa of Fabricius, and spleen were evaluated in chickens infected with strains representing variation in the level of virulence, namely, avirulent CP5, virulent CP18, and a relatively more virulent CP26 strain. Immunophenotyping analysis showed that CT or splenic macrophage frequencies were significantly higher in CP18- and CP26-infected chickens compared with uninfected controls, while the frequencies of gamma delta T-cells and B-cells in the CT of CP26-infected chickens were significantly higher than those in the uninfected, CP5- or CP18-infected groups. The T-cell analysis showed that chickens infected with CP18 and CP26 had a significantly higher number of splenic CD4(+) and CD8(+) T-cells expressing CD44 and CD28 activation molecules, while CP26-infected chickens also had significantly increased CT frequency of these activated CD4(+) and CD8(+) T-cells when compared with uninfected or CP5-infected groups. Collectively, our findings suggested that cellular responses, including activation of T-cells, are selectively induced against virulent CP strains and that the NE-producing characteristics of this pathogen may influence the outcome of immunity to NE.
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页码:186 / 196
页数:11
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