Multi-point immobilization of GH 11 endo-13-1,4-xylanase on magnetic MOF composites for higher yield of xylo-oligosaccharides

被引：8

作者：

Gui, Yifan ^{[1
,2
]}

Wu, Ya ^{[1
,2
]}

Shu, Tong ^{[1
,2
]}

Hou, Ziqi ^{[1
,2
]}

Hu, Yaofeng ^{[1
,2
]}

Li, Wei ^{[1
,2
]}

Yu, Longjiang ^{[1
,2
]}

机构：

[1] Huazhong Univ Sci & Technol, Inst Resource Biol & Biotechnol, Coll Life Sci & Technol, Dept Biotechnol, Wuhan 430074, Peoples R China

[2] Huazhong Univ Sci & Technol, Key Lab Mol Biophys, Minist Educ, Wuhan 430074, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2024年 / 260卷

关键词：

Xylo-oligosaccharides; Immobilized xylanases; Metal-organic frameworks (MOFs); Polydopamine (PDA); ENDO-XYLANASE; NANOPARTICLES; XYLOOLIGOSACCHARIDES; EFFICIENT;

D O I：

10.1016/j.ijbiomac.2024.129277

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

GH 11 endo-13-1,4-xylanase (Xy) was a crucial enzyme for xylooligosaccharides (XOS) production. The lower reusability and higher cost of purification has limited the industrial application of Xy. Addressing these challenges, our study utilized various immobilization techniques, different supports and forces for Xy immobilization. This study presents a new method in the development of Fe3O4@PDA@MOF-Xy which is immobilized via multipoint interaction forces, demonstrating a significant advancement in protein loading capacity (80.67 mg/g), and exhibiting remarkable tolerance to acidic and alkaline conditions. This method significantly improved Xy reusability and efficiency for industrial applications, maintaining 60 % activity over 10 cycles. Approximately 23 % XOS production was achieved by Fe3O4@PDA@MOF-Xy. Moreover, the yield of XOS from cobcorn xylan using this system was 1.15 times higher than that of the free enzyme system. These results provide a theoretical and applicative basis for enzyme immobilization and XOS industrial production.

引用

页数：13