Effects of exogenous amino acids on the freeze-drying survival rate of Lactiplantibacillus plantarum LIP-1

被引：2
|
作者：
Yang, Youxin [1 ,2 ,3 ]
Wang, Ruixue [1 ,2 ,3 ]
Chen, Zichao [1 ,2 ,3 ]
Yang, Ying [1 ,2 ,3 ]
Jingjing, E. [1 ,2 ,3 ]
Gao, Aiwu [3 ]
Wang, Junguo [1 ,2 ,3 ]
机构：
[1] Educ Minist PR China, Key Lab Dairy Biotechnol & Engn, Hohhot, Peoples R China
[2] Minist Agr & Rural Affairs, Key Lab Dairy Prod Proc, Hohhot, Peoples R China
[3] Inner Mongolia Agr Univ, Coll Food Sci & Engn, Hohhot, Peoples R China
来源：
DRYING TECHNOLOGY | 2024年 / 42卷 / 04期
基金：
中国国家自然科学基金;
关键词：
Lactiplantibacillus plantarum LIP-1; glycine; freeze-drying survival rate; cell wall; cell membrane; DNA; LACTOBACILLUS-ACIDOPHILUS; FATTY-ACIDS; CELL-WALL; VIABILITY; BACTERIA; CRYOPROTECTANTS; PEPTIDOGLYCAN; CONSTITUENTS; METABOLISM; TOLERANCE;
D O I：
10.1080/07373937.2023.2297265
中图分类号：
TQ [化学工业];
学科分类号：
0817 ;
摘要：
How to improve the freeze-drying survival rate of Lactic acid bacteria (LAB) by increasing its resistance is a problem that is worth paying attention to. We evaluated the effect of exogenous amino acids on freeze-drying resistance of strain using Lactiplantibacillus plantarum LIP-1 as model probiotics. We found that the survival rate of L. plantarum LIP-1 after freeze-dried could be significantly enhanced by adding 0.04 g/L glycine to medium (p < 0.05), compare with other amino acids. Mechanistic studies showed that the content of peptidoglycan was increased to improve the integrity of the cell wall via metabolizing glycine; By metabolizing glycine, the content of unsaturated fatty acid was increased, such as C19cyc11, to promote membrane fluidity. In addition, the metabolism of glycine could neutralize the acidic intracellular environment to reduce DNA damage, thus improve the survival rate of freeze-dried strain. These findings may provide a new idea for industrialization of LAB powder.
引用
收藏
页码：650 / 660
页数：11
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