Nano-bio interaction between human immunoglobulin G and nontoxic, near-infrared emitting water-borne silicon quantum dot micelles

被引：4

作者：

Chinnathambi, Shanmugavel ^{[1
,2
]}

Shirahata, Naoto ^{[3
,4
,5
]}

Kumar, Mahima ^{[1
]}

Karthikeyan, Subramani ^{[6
]}

Abe, Katsuhiko ^{[1
]}

Thangavel, Vaijayanthi ^{[1
]}

Pandian, Ganesh N. ^{[1
]}

机构：

[1] Kyoto Univ, Inst Integrated Cell Mat Sci WPI iCeMS, Inst Adv Study, Kyoto 6068501, Japan

[2] Natl Inst Mat Sci NIMS, Int Ctr Young Scientists, 1-2-1 Sengen, Tsukuba, Ibaraki 3050047, Japan

[3] Hokkaido Univ, Grad Sch Chem Sci & Engn, Sapporo 0600814, Japan

[4] NIMS, Int Ctr Mat Nanoarchitecton WPI MANA, Namiki, Tsukuba 3050044, Japan

[5] Chuo Univ, Dept Phys, 1-13-27 Kasuga,Bunkyo, Tokyo 1128551, Japan

[6] Vellore Inst Technol, Ctr Healthcare Advancement Innovat & Res, Chennai 600127, India

来源：

RSC ADVANCES | 2023年 / 13卷 / 09期

关键词：

NONSPECIFIC INTERACTION; SILVER NANOPARTICLES; ACCURATE DOCKING; NANOCRYSTALS; CONFORMATION; FLUORESCENCE; TOXICITY; GLIDE; IGG;

D O I：

10.1039/d3ra00552f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In recent years, the field of nanomaterials has exponentially expanded with versatile biological applications. However, one of the roadblocks to their clinical translation is the critical knowledge gap about how the nanomaterials interact with the biological microenvironment (nano-bio interactions). When nanomaterials are used as drug carriers or contrast agents for biological imaging, the nano-bio interaction-mediated protein conformational changes and misfolding could lead to disease-related molecular alterations and/or cell death. Here, we studied the conformation changes of human immunoglobulin G (IgG) upon interaction with silicon quantum dots functionalized with 1-decene, Pluronic-F127 (SiQD-De/F127 micelles) using UV-visible, fluorescence steady state and excited state kinetics, circular dichroism, and molecular modeling. Decene monolayer terminated SiQDs are accumulated inside the Pluronic F127 shells to form SiQD-De/F127 micelles and were shown to bind strongly with IgG. In addition, biological evaluation studies in cell lines (HeLa, Fibroblast) and medaka fish (eggs and larvae) showed enhanced uptake and minimal cytotoxicity. Our results substantiate that engineered QDs obviating the protein conformational changes could have adept bioefficacy.

引用

页码：6051 / 6064

页数：14