Linearly modulated multi-focal diffractive lens for multi-sheet excitation of flow driven samples in a light-sheet fluorescence microscope

被引：2
|
作者：
Hofmann, Meike [1 ]
Ghebjagh, Shima Gharbi [1 ]
Feng, Yuchao [1 ]
Fan, Chao [1 ]
Lemke, Karen [2 ]
Sinzinger, Stefan [1 ]
机构：
[1] Tech Univ Ilmenau, Fachgebiet Tech Opt, Postfach 100565, D-98684 Ilmenau, Germany
[2] Iba Heiligenstadt, D-37308 Heilbad Heiligenstadt, Germany
来源：
JOURNAL OF THE EUROPEAN OPTICAL SOCIETY-RAPID PUBLICATIONS | 2023年 / 19卷 / 01期
关键词：
Light sheet fluorescence microscopy; Diffractive optical elements; Multi-plane imaging; 3D imaging; Multi-focal lens;
D O I：
10.1051/jeos/2023022
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Light sheet fluorescence microscope with single light sheet illumination enables rapid 3D imaging of living cells. In this paper we show the design, fabrication and characterization of a diffractive optical element producing several light sheets along a 45 degrees inclined tube. The element, which is based on a multi-focal diffractive lens and a linear grating, generates five thin light sheets with equal intensities when combined with a refractive cylindrical lens. The generated uniform light sheets can be applied for the scanning of samples in tubes enabling flow-driven 3-dimensional imaging.
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页数：8
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