RESONANCE ENERGY-TRANSFER;
GREEN FLUORESCENT PROTEINS;
DESIGN PRINCIPLES;
SMALL MOLECULES;
IN-VIVO;
KINASE;
VOLTAGE;
CAMP;
OPTIMIZATION;
EXPRESSION;
D O I:
10.1039/d2cc05363b
中图分类号:
O6 [化学];
学科分类号:
0703 ;
摘要:
Genetically-encoded biosensors provide the all-optical and non-invasive visualization of dynamic biochemical events within Living systems, which has allowed the discovery of profound new insights. Twenty-five years of biosensor development has steadily improved their performance and has provided us with an ever increasing biosensor repertoire. In this feature article, we present recent advances made in biosensor development and provide a perspective on the future direction of the field.
机构:
Department of Life Sciences, Imperial College London, London SW7 2AZ, United Kingdom
Centre for Synthetic Biology and Innovation, Imperial College London, London SW7 2AZ, United KingdomDepartment of Life Sciences, Imperial College London, London SW7 2AZ, United Kingdom
Polizzi, Karen M
Kontoravdi, Cleo
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机构:
Department of Chemical Engineering and Chemical Technology, Imperial College London, London SW7 2AZ, United KingdomDepartment of Life Sciences, Imperial College London, London SW7 2AZ, United Kingdom
机构:
Univ London Imperial Coll Sci Technol & Med, Dept Life Sci, London SW7 2AZ, England
Univ London Imperial Coll Sci Technol & Med, Ctr Synthet Biol & Innovat, London SW7 2AZ, EnglandUniv London Imperial Coll Sci Technol & Med, Dept Life Sci, London SW7 2AZ, England
Polizzi, Karen M.
Kontoravdi, Cleo
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机构:
Univ London Imperial Coll Sci Technol & Med, Dept Chem Engn & Chem Technol, London SW7 2AZ, EnglandUniv London Imperial Coll Sci Technol & Med, Dept Life Sci, London SW7 2AZ, England
机构:
E China Univ Sci & Technol, Dept Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R ChinaE China Univ Sci & Technol, Dept Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
Zhang, Chang
Ye, Bang-Ce
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机构:
E China Univ Sci & Technol, Dept Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R ChinaE China Univ Sci & Technol, Dept Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
Ye, Bang-Ce
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY,
2014,
247
机构:
College of Chemistry and Molecular Engineering, Peking University
Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, PKU-IDG/Mc Govern Institute for Brain Research, Peking UniversityCollege of Chemistry and Molecular Engineering, Peking University
Luxin Peng
Yongxian Xu
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机构:
Peking-Tsinghua Center for Life Sciences, Peking University
Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, PKU-IDG/Mc Govern Institute for Brain Research, Peking UniversityCollege of Chemistry and Molecular Engineering, Peking University
Yongxian Xu
Peng Zou
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h-index: 0
机构:
College of Chemistry and Molecular Engineering, Peking University
Peking-Tsinghua Center for Life Sciences, Peking University
Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, PKU-IDG/Mc Govern Institute for Brain Research, Peking UniversityCollege of Chemistry and Molecular Engineering, Peking University