Smart genetically-encoded biosensors for the chemical monitoring of living systems

被引:3
|
作者
Van Thillo, Toon [1 ]
Van Deuren, Vincent [1 ]
Dedecker, Peter [1 ]
机构
[1] Katholieke Univ Leuven, Dept Chem, Celestijnenlaan 200G, B-3001 Leuven, Belgium
基金
欧洲研究理事会;
关键词
RESONANCE ENERGY-TRANSFER; GREEN FLUORESCENT PROTEINS; DESIGN PRINCIPLES; SMALL MOLECULES; IN-VIVO; KINASE; VOLTAGE; CAMP; OPTIMIZATION; EXPRESSION;
D O I
10.1039/d2cc05363b
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Genetically-encoded biosensors provide the all-optical and non-invasive visualization of dynamic biochemical events within Living systems, which has allowed the discovery of profound new insights. Twenty-five years of biosensor development has steadily improved their performance and has provided us with an ever increasing biosensor repertoire. In this feature article, we present recent advances made in biosensor development and provide a perspective on the future direction of the field.
引用
收藏
页码:520 / 534
页数:15
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