ApoE通过影响mir-30a-5p调控BDNF表达

被引：0

作者：

臧贵勇 ^{[1
]}

潘开昌 ^{[1
]}

龙友国 ^{[2
]}

余跃生 ^{[3
]}

李成朋 ^{[4
,5
]}

官志忠 ^{[4
,5
]}

禹文峰 ^{[4
,5
]}

机构：

[1] 黔南民族医学高等专科学校解剖学与组织胚胎学教研室

[2] 黔南民族医学高等专科学校生物化学教研室

[3] 黔南民族医学高等专科学校预防医学教研室

[4] 地方病与少数民族疾病教育部重点实验室(贵州医科大学)

[5] 贵州医科大学分子生物学重点实验室

来源：

中风与神经疾病杂志 | 2018年 / 35卷 / 10期

关键词：

阿尔茨海默病; ApoE; 神经营养因子; mir-30a-5p;

D O I：

10.19845/j.cnki.zfysjjbzz.2018.10.007

中图分类号：

R749.16 [];

学科分类号：

100203 ;

摘要：

目的研究ApoE基因型是如何影响脑源性神经营养因子(brain derived neurotrophic factor,BDNF)的表达,以更好的理解AD的发病机制。方法在SH-SY5Y细胞系和大鼠原代神经元中加入Apo E3和Apo E4重组蛋白,用real-time PCR、Western blot和ELISA检测BDNF的表达水平;用luciferase assay、real-time PCR和Western blot证实BDNF是mir-30a-5p的靶基因;用Taq Man技术检测mir-30a-5p的水平。结果与对照组相比,Apo E3增加了BDNF的mRNA、蛋白水平和培养基中的BDNF浓度,而ApoE4减少了BDNF的mRNA、蛋白水平和培养基中的BDNF浓度; hsa-mir-30a-5p抑制BDNF的3’UTR的luciferase活性,mRNA和蛋白水平。ApoE3降低mir-30a-5p的表达,Apo E4增加mir-30a-5p的表达,而过表达mir-30a-5p inhibitor可以逆转ApoE4对BDNF的抑制效果。结论BDNF是mir-30a-5p的靶基因,ApoE是通过影响mir-30a-5p的水平来调控BDNF的表达。

引用

页码：893 / 896

页数：4

共 10 条

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