PCR-SSCP检测胃癌中p53基因突变

被引：1

作者：

苏琦

何冬梅

梁晓秋

宋颖

李一琴

机构：

[1] 南华大学肿瘤研究所

来源：

南华大学学报(医学版) | 2001年 / 06期

关键词：

胃肿瘤; p53基因; 表达; 突变;

D O I：

暂无

中图分类号：

R735.2 [胃肿瘤];

学科分类号：

摘要：

目的　研究p5 3基因表达及突变在胃癌发生中的地位。方法　采用链霉菌抗生物素蛋白 -过氧化酶 (S -P)法与聚合酶链反应 -单链构象多态性 (PCR -SSCP)分析技术分别检测胃癌p5 3基因表达及突变。结果　p5 3蛋白表达在正常胃粘膜均为阴性 (0 / 2 0 ) ,在不典型增生为 18% (11/ 6 0 ) ,其中轻度全为阴性(0 / 2 0 ) ,中度 2 5 % (5 / 2 0 ) ,重度 30 % (6 / 2 0 ) ,明显低于胃癌的 5 8% (70 / 12 0 ) (P <0 .0 5 ) ,而中度、重度不典型增生高于正常胃粘膜及轻度不典型增生 (P <0 .0 5 )。胃癌中p5 3基因第 5外显子突变率高达 2 5 % (5 / 2 0 ) ,显著高于无突变的正常胃粘膜 0 % (0 / 2 0 ) (P <0 .0 5 )。结论　p5 3基因突变可出现于中度、重度不典型增生 ,是胃癌发生的一个早期事件。在胃癌发生中p5 3基因第 5外显子突变频繁

引用

页码：542 / 545+548 +548

页数：5

共 10 条

[1] p53 gene mutation in gastric and esophageal cancers. Imazeki F,Omata M,Nose H,et al. Gastroenterology . 1992
[2] Alteration of p53 gene in primary gastric cancer tissues. Hong SI,Hong WS,Jang JJ,et al. Anticancer Research . 1994
[3] Expression of p53 in early gastric carcinoma and precancerous edj edjacent mucosa. Brito MJ,Willians GT,Thampson H,et al. Gut . 1994
[4] Genetic alteration in human gastrointestnal cancers. Tahara E. Cancer . 1995
[5] Alterations of (CA)n DNA repeats and tumor supressor genes in human gastric cancer. Mironov NM,Aguebon MA,Potapova GI,et al. Cancer Research . 1994
[6] Chronology of p53 protein accumulation in gastric carcinogenesis. Craanen ME,Blok P,Dekkerx W,et al. Gut . 1995
[7] p53 mutation in gastric cancer: a genetic model for carcinogenesis is common to gastric and colorectal cancer. Uchino S,Noguchi M,Ochiai A,et al. International Journal of Cancer . 1993
[8] p53 alteration on gastric precancerous lesions. Shiao YH,Rugge M,Correa P,et al. American Journal of Pathology . 1994
[9] The p53 tumor suppressor gene. Levine AJ,Momand J,Finaly CA,et al. Nature . 1991
[10] p53 gene mutation and protein nuclear accumulation are early events in intestinal type gastric cancer but late events in diffuse type. Ranzani GN,Lwinettio,Padovan LS,et al. Cancer Epidemiology Biomarkers and Prevention . 1995

← 1 →