Random amplified polymorphic DNA analysis of eel genome

被引:0
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作者
QIU JIA
JING
YI PING LI(Shanghai Institute of Cell Biology Chincsc Acadcmy of Sci-ences) 320 Yue Yang Road
机构
关键词
Eel; RAPD; pojymrphic fragments; genetic distance;
D O I
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中图分类号
Q52 [核酸];
学科分类号
摘要
Eel family is a huge one, in which many kinds of eelsespecially some migratory eels, bear strong resemblance toeach other, and are therefOre difficult to be identified. Inthis study 29 random primers were used to make RAPDana1ysis for Japanese ee1 (Angui11ajaponica). European eel(Anguilla anguilla) and Pike eel (AIllrae1lesox cinereus).And totally 299 fragments were counted. Shared or spe-cific fragments were counted alld ge11etic similarity or ge-netic distance were calculated. The genetic similarity be-tween Japanese eel alld Pike eel is 0.68 and the geneticdistance between them is 0.32; those between Europeaneel and Pike eel are 0.72 and 0.28 respectively, and be-tween Japanese eel and Ellropean eel are 0.74 and 0.25 re-spectively. The method has been shown to be suitable tomolecular identification of eels. It provides an a1ternativeapproach to determine the relatiollship between species.
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页码:217 / 223
页数:7
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