Aquaporin-1 Expressed in Cultured Human Trabecular Meshwork Cells

被引：0

作者：

Mingkai Lin Jian Ge Yehong Zhuo Yuqing Lan Keming Yu Jianliang ZhengZhongshan Ophthalmic center Sun Yatsen University Guangzhou China ^{[510060
]}

机构：

来源：

眼科学报 | 2002年 / 01期

关键词：

aquaporin-1; glaucoma; trabecular meshwork cell;

D O I：

暂无

中图分类号：

R770.2 [];

学科分类号：

摘要：

<正>Objective: To determine if aquaporin-1 could be detected in cultures of human trabecularmeshwork cells.Methods: Using primers specific for aquaporin-1, reverse transcription combined withpolymerase chain reaction (RT-PCR) yielded a product and its size with total RNAprepared from the human trabecular meshwork cells. SDS-PAGE and immunoblottingwere also used in this study to detect the specific water channel.Results: The presence of this product and its size (298 base pairs) are consistent withthat of an aquaporin-1 message in these cells. A band of 28 kD in agreement with themolecular size of aquaporin-1 was showed in a film by immunoblotting.Conclusion: The presence of aquaporin-1 in human trabecular meshwork cells, thepredominant cell-type of the primary outflow region of the human eye, suggests that waterchannels may be involved in the movement of aqueous fluid out of the eye. In addition,the existence of aquaporin-1 on cultures of human trabecular meshwork cells provides anin vitro model to study

引用

页码：33 / 36

页数：4

共 8 条

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