miR-424-5p靶向ARK5逆转胃癌耐药细胞对顺铂的耐药性的影响及机制研究

被引：0
|
作者：
杨光
武鸣霞
机构：
[1] 铁岭市中心医院
来源：
世界临床药物 | 2021年 / 42卷 / 12期
关键词：
胃癌; miR-424-5p; ARK5; 顺铂; 耐药性;
D O I：
10.13683/j.wph.2021.12.009
中图分类号：
R735.2 [胃肿瘤];
学科分类号：
摘要：
目的探讨miR-424-5p对胃癌细胞顺铂(cisplatin,DDP)耐药的影响及其作用机制。方法利用RT-qPCR检测胃癌DDP耐药细胞(SGC7901/DDP)及其亲代细胞(SGC7901)中miR-424-5p及ARK5的表达水平;利用Lipofectamine 2000将miR-424-5p mimic、siR-ARK5、miR-424-5p mimic+pcDNA-ARK5转染至SGC7901/DDP中,通过MTT法测定各转染组SGC7901/DDP细胞对DDP耐药性的影响;以Target Scan预测miR-424-5p的潜在靶基因,采用双荧光素酶试验进行验证;利用免疫印迹(western blot,WB)试验测定转染后ARK5蛋白的表达水平。结果胃癌DDP耐药细胞中miR-424-5p表达显著下调,ARK5表达显著上调;MTT试验显示,过表达miR-424-5p及干扰ARK5表达降低了DDP耐药细胞的耐药性,共同转染miR-424-5p mimic和pcDNA-ARK5逆转了miR-424-5p mimic单独转染对胃癌细胞耐药性的影响;Target Scan预测发现,ARK5是miR-424-5p的潜在靶基因,双荧光素酶试验证实ARK5是miR-424-5p的靶基因;Western-blot试验结果显示miR-424-5p负向调控ARK5蛋白的表达。结论胃癌DDP耐药细胞中miR-424-5p表达下调,ARK5表达上调;miR-424-5p通过靶向抑制ARK5表达有效逆转胃癌DDP耐药细胞的耐药性。
引用
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页码：1073 / 1080
页数：8
相关论文
共 19 条
  • [1] 中国癌症态势七十年分析
    邹小农
    赵平
    [J]. 中国肿瘤临床与康复, 2019, 26 (10) : 1153 - 1161
  • [2] K562细胞及其耐药细胞株K562/A02细胞白血病耐药相关microRNA筛选
    曹翊雄
    戴崇文
    张广森
    [J]. 中华血液学杂志, 2010, (06) : 361 - 365
  • [3] miR-424-5p Regulates Hepatoma Cell Proliferation and Apoptosis[J] . Piao Lianshu,Wang Fei,Wang Yanyan,Yang Zirong,Li Qianwei,Cui Lifeng,Yu Qinggong.Cancer biotherapy & radiopharmaceuticals . 2019 (3)
  • [4] miR-424-5p regulates cell proliferation, migration and invasion by targeting doublecortin-like kinase 1 in basal-like breast cancer[J] . Jianling Wang,Shibing Wang,Jijun Zhou,Qian Qian.Biomedicine & Pharmacotherapy . 2018
  • [5] Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries
    Bray, Freddie
    Ferlay, Jacques
    Soerjomataram, Isabelle
    Siegel, Rebecca L.
    Torre, Lindsey A.
    Jemal, Ahmedin
    [J]. CA-A CANCER JOURNAL FOR CLINICIANS, 2018, 68 (06) : 394 - 424
  • [6] Inhibition of AMPK-related kinase 5 (ARK5) enhances cisplatin cytotoxicity in non-small cell lung cancer cells through regulation of epithelial-mesenchymal transition
    Li, Minghui
    Zheng, Chengfei
    Xu, Hongfei
    He, Wei
    Ruan, Yongchun
    Ma, Jianyong
    Zheng, Junnan
    Ye, Chengmeng
    Li, Weidong
    [J]. AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH, 2017, 9 (04): : 1708 - 1719
  • [7] Salinomycin enhances doxorubicin sensitivity through reversing the epithelial-mesenchymal transition of cholangiocarcinoma cells by regulating ARK5
    Yu, Z.
    Cheng, H.
    Zhu, H.
    Cao, M.
    Lu, C.
    Bao, S.
    Pan, Y.
    Li, Y.
    [J]. BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH, 2017, 50 (10)
  • [8] Loss of MiR-424-3p, Not miR-424-5p, Confers Chemoresistance Through Targeting YAP1 in Non-small Cell Lung Cancer
    Zhang, Meichun
    Zeng, Jun
    Zhao, Ziwen
    Liu, Zhaohui
    [J]. MOLECULAR CARCINOGENESIS, 2017, 56 (03) : 821 - 832
  • [9] miR424-5p functions as an anti-oncogene in cervical cancer cell growth by targeting KDM5B via the Notch signaling pathway[J] . Yan Zhou,Qi An,Rui-xia Guo,Yu-huan Qiao,Liu-xia Li,Xiao-yan Zhang,Xian-lan Zhao.Life Sciences . 2017
  • [10] miR-424 acts as a tumor radiosensitizer by targeting aprataxin in cervical cancer
    Wang, Xia
    Li, Qing
    Jin, Hua
    Zou, Hua
    Xia, Wei
    Dai, Nan
    Dai, Xiao-Yan
    Wang, Dong
    Xu, Cheng-Xiong
    Qing, Yi
    [J]. ONCOTARGET, 2016, 7 (47) : 77508 - 77515
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