Study on Long-Terms Survival and Gene Expression of pSVPoMcat Genetically Modified Schwann Cell in the Rat Spinal Cord Injure

Objective To observe the survival and gene expression of genetically modified schwann cell (SC) with pSVPoMcat in the rat spinal cord.Methods The experimental animals were divided into three groups randomly, that is, pSVPoMcat modified SC implantation group(Group A),highly purified SC implantation group(Group B),and the controlled group (Group C). After operation, the survival time for each group was 2, 4, 8, 12 weeks respectively, 10 rats once for each group, then the section of spinal cord grafted were checked by immunocytochemical staining of S－100 protein, myelin basic protein(MBP) and in situ hybridization.Results After operation for 2, 4. 8. 12 weeks, Group A' S－100, MBP staining cells and ISH cells were respectively (82.3± 6.07)％ , (77.8± 12.3)％ , (67.8± 8.6)％； (81.2± 5.2)％ , (76.3± 11.8)％ , (68.4± 8.3)％； (80.7± 5.6)％ , (75.4± 11.6)％ ,(66.4± 8.2)％； (81.6± 6.2)％ , (75.8± 9.5)％ , (63.3± 8.02)％ ,(P<0.05). Among them, We can find that myelin sheath emerges in ISH positive cells Group B'S S－100 staining cells are (66.3± 6.8)％ , (40.2± 11.2)％ , (10.8± 2.6)％ , (3.2± 0.6)％ ,(P< 0.001), but no MBP staining cells and ISH cells are found. And in Group C, no positive cells are found at all.Conclusion pSVPoMcat genetically modified SC can survive for a long time and express gene in intraspinal injured and help to formation myelin of spinal cord injured (SCI) which provide a feasible way to gene care to spinal injury.