MiR-367通过Wnt/β-catenin通路抑制成骨细胞增殖的研究

被引：6
|
作者：
马建国
高志明
靳雷
机构：
[1] 南部战区海军第二医院骨科
来源：
实用骨科杂志 | 2020年 / 26卷 / 01期
关键词：
成骨细胞; 细胞增殖; 细胞分化; miR-367; Wnt/β-catenin通路;
D O I：
10.13795/j.cnki.sgkz.2020.01.009
中图分类号：
R580 [];
学科分类号：
摘要：
目的探究MiR-367通过Wnt/β-连环蛋白(β-catenin)通路对成骨细胞增殖的抑制作用。方法培养骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)后转染miR-367模拟物或抑制物,茜素红染色观察成骨分化;培养新生大鼠成骨细胞,转染miR-367模拟物或抑制物、联合使用β-catenin激动剂SKL2001或抑制剂XAV939,采用MTT法检测细胞增殖活力,采用荧光定量PCR及Western blot检测β-catenin及成骨标志基因Runt2相关转录因子2(Runt-related transcription factor 2,RUNX2)、碱性磷酸酶(alkaline phosphatase,ALP)、骨钙素(osteocalcin,OCN)、Ⅰ型胶原(Collagen-Ⅰ,Col-Ⅰ)的表达。结果转染miR-367模拟物或抑制物后,BMSCs茜素红染色的OD570值与转染NC模拟物或抑制物比较差异无统计学意义(P>0.05);转染miR-367模拟物后新生大鼠成骨细胞MTS检测的OD490值及β-catenin、RUNX2、ALP、OCN、Col-Ⅰ的表达水平明显低于转染NC模拟物,转染miR-367抑制物后新生大鼠成骨细胞MTS检测的OD490值及β-catenin、RUNX2、ALP、OCN、Col-Ⅰ的表达水平明显高于转染NC抑制物(P<0.05);转染miR-367模拟物联合SKL2001处理后新生大鼠成骨细胞中β-catenin、RUNX2、ALP、OCN、Col-Ⅰ的表达水平明显高于转染miR-367模拟物,转染miR-367抑制物联合XAV939处理后新生大鼠成骨细胞中β-catenin、RUNX2、ALP、OCN、Col-Ⅰ的表达水平明显低于转染miR-367抑制物。结论 miR-367能够抑制成骨细胞的增殖、但不影响成骨细胞的分化,这一作用可能与抑制Wnt/β-catenin通路有关。
引用
收藏
页码：34 / 39
页数：6
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