Application of Metabolomics Approach to Study on Chemical Constituents in Cultivated and Wild Pinellia ternate （Thunb.） Breit. and Typhonium flagelliforme （Lodd.） Blume Based on UPLC-ESI-HRMSn

被引：1

作者：

Yanfang LI ^{[1
]}

Gang KUANG ^{[2
]}

Ainuan LI ^{[1
]}

Junke CHEN ^{[1
]}

Hui SUN ^{[3
]}

Rui ZENG ^{[1
]}

Xin LI ^{[3
]}

机构：

[1] College of Pharmacy,Southwest Minzu University

[2] Department of Biological & Chemical Engineering,Chongqing University of Education

[3] Department of Environmental Science and Engineering,Sichuan University

来源：

Medicinal Plant | 2019年 / 01期

关键词：

Pinellia ternate(Thunb.) Breit; Plant metabolomics; Marker; UPLC-ESI-HRMSn; PCA; OPLS-DA;

D O I：

10.19600/j.cnki.issn2152-3924.2019.01.006

中图分类号：

R284.1 [化学分析与鉴定];

学科分类号：

1008 ;

摘要：

[Objectives] The aim was to study chemical components of cultivated and wild Pinellia ternate（Thunb.） Breit and Typhonium flagelliforme（Lodd.） Blume by metabolomics technology based on UPLC-ESI-HRMS;. [Methods] Chemical components of 27 samples of cultivated and wild P. ternate and T. flagelliforme were detected by using UPLC-ESI-HRMS;technology. Differential markers between P. ternate and T. flagelliforme as well as cultivated and wild P. ternate were screened and identified by principal component analysis（PCA） and orthogonal partial least square-discriminate analysis（OPLS-DA）. [Results]12 components of P. ternate（cultivated and wild variety） and T.flagelliforme and three components of cultivated and wild P. ternate were screened and identified by OPLS-DA. N-Acetyl-6-hydroxy-L-tryptophan and N-Phenylacetylglutamic were not detected in P. ternate,and these two components can be used as potential markers of P. ternate and T. flagelliforme. [Conclusions]UPLC-ESI-HRMS;-based metabolomics technology could be used to quickly identify the chemical constituents of P. ternate and effectively distinguish P. ternate and T. flagelliforme,which has reference significance for the quality control of P. ternate.

引用

页码：19 / 22 +25

页数：5