Development of a sensitive and reliable droplet digital PCR assay for the detection of ‘Candidatus Liberibacter asiaticus'

被引：0

作者：

ZHONG Xi ^{[1
]}

LIU Xue-lu ^{[1
]}

LOU Bing-hai ^{[2
]}

ZHOU Chang-yong ^{[1
]}

WANG Xue-feng ^{[1
]}

机构：

[1] National Citrus Engineering Research Center, Citrus Research Institute, Chinese Academy of Agricultural Sciences/Southwest University

[2] Guangxi Key Laboratory of Citrus Biology, Guangxi Academy of Specialty Crops

来源：

JournalofIntegrativeAgriculture | 2018年 / 17卷 / 02期

关键词：

citrus Huanglongbing; early diagnosis; droplet digital PCR;

D O I：

暂无

中图分类号：

S436.66 [柑桔类病虫害];

学科分类号：

摘要：

Citrus Huanglongbing(HLB, yellow shoot disease) is one of the most serious citrus diseases worldwide. To better improve the detection sensitivity, a droplet digital PCR(ddPCR) assay was developed for the rapid detection of ‘Candidatus Liberibacter asiaticus’(Las), the putative causal agent of HLB. The detection of sensitivity comparison using positive plasmid indicated that dd PCR was superior to quantitative PCR(qPCR) for detecting and quantifying Las at low concentrations. The Las detection of 40 field samples also showed that six of 13 asymptomatic samples(46.15%) with high Ct value(>35) were positive by dd PCR. This methodology showed great potential for early HLB infection diagnosis.

引用

页码：483 / 487

页数：5

共 50 条

[1] Development of a sensitive and reliable droplet digital PCR assay for the detection of 'Candidatus Liberibacter asiaticus'
Zhong Xi
Liu Xue-lu
Lou Bing-hai
Zhou Chang-yong
Wang Xue-feng
JOURNAL OF INTEGRATIVE AGRICULTURE, 2018, 17 (02) : 483 - 487
[2] Development of a duplex droplet digital PCR assay for absolute quantitative detection of "Candidatus Liberibacter asiaticus"
Selvaraj, Vijayanandraj
Maheshwari, Yogita
Hajeri, Subhas
Chen, Jianchi
McCollum, Thomas Greg
Yokomi, Raymond
PLOS ONE, 2018, 13 (05):
[3] Multiplex detection of "Candidatus Liberibacter asiaticus" and Spiroplasma citri by qPCR and droplet digital PCR
Maheshwari, Yogita
Selvaraj, Vijayanandraj
Godfrey, Kristine
Hajeri, Subhas
Yokomi, Raymond
PLOS ONE, 2021, 16 (03):
[4] Development of efficient and reliable Candidatus Liberibacter asiaticus detection methods in citrus
Vazquez, O.
Chavez, S.
Kunta, M.
Braswell, E.
Keremane, M. L.
Lee, R. F.
Louzada, E. S.
PHYTOPATHOLOGY, 2014, 104 (11) : 121 - 121
[5] An Improved Reference Gene for Detection of "Candidatus Liberibacter asiaticus" Associated with Citrus Huanglongbing by qPCR and Digital Droplet PCR Assays
Keremane, Manjunath L.
McCollum, Thomas G.
Roose, Mikeal L.
Lee, Richard F.
Ramadugu, Chandrika
PLANTS-BASEL, 2021, 10 (10):
[6] Optimization of vqPCR for Reliable Detection of Viable Candidatus Liberibacter asiaticus in Citrus
Louzada, Eliezer
Vazquez, Omar
Chavez, Sandy
Setamou, Mamoudou
Kunta, Madhurababu
HORTSCIENCE, 2022, 57 (06) : 692 - 697
[7] Establishment of a Sensitive and Reliable Droplet Digital PCR Assay for the Detection of Bursaphelenchus xylophilus
Su, Yu
Zhu, Xuedong
Jing, Haozheng
Yu, Haiying
Liu, Huai
PLANTS-BASEL, 2024, 13 (19):
[8] Detection of 'Candidatus Liberibacter asiaticus' by recombinase polymerase amplification (RPA) assay
Park, J. W.
Kunta, M.
Da Graca, J. V.
Braswell, W. E.
PHYTOPATHOLOGY, 2020, 110 (12) : 12 - 12
[9] Development of a droplet digital PCR assay for the sensitive detection of iridovirus in Andrias davidianus
Meng, Yan
Jiang, Nan
Xie, Yixing
Wei, Ying
Wang, Cheng
Tian, Mingzhu
Xue, Mingyang
Xu, Chen
Li, Yiqun
Liu, Wenzhi
Fan, Yuding
Zhou, Yong
JOURNAL OF FISH DISEASES, 2023, 46 (11) : 1249 - 1256
[10] Development of a droplet digital PCR assay for sensitive detection of porcine circovirus 3
Zhang, Yongning
Zhang, Zhou
Wang, Zhanying
Wang, Zili
Wang, Caixia
Feng, Chunyan
Yuan, Wanzhe
Lin, Xiangmei
Wu, Shaoqiang
MOLECULAR AND CELLULAR PROBES, 2019, 43 : 50 - 57

← 1 2 3 4 5 →