Cloning and Expression Analysis of a Mitogen-Activated Protein Kinase Gene OsMPK14 in Rice附视频

Mitogen activated-protein kinases(MAPKs)are important components in signal transduction pathways responding to various biotic and abiotic stresses.An MAPK gene,OsMPK14(GenBank Accession No.GQ265780)from rice(Oryza sativa L.),was cloned by RT-PCR.The full-length cDNA of OsMPK14 consists of 1660 bp in size,containing an open reading frame of 1629 bp,which encodes a 542-amino-acid polypeptide and has a typical protein kinase domain and a phosphorylation activation motif TDY.Sequence alignment and analysis revealed that OsMPK14 was located on rice chromosome 5,and composed of nine exons and eight introns in the coding region.Semi-quantitative RT-PCR was performed to detect the expression patterns of OsMPK14 in rice shoots and roots under darkness,drought,high salinity,low temperature and abscisic acid treatments.The OsMPK14 mRNA was induced by abscisic acid,low temperature and high salinity,but weakly inhibited by drought.In addition,the expression of OsMPK14 was up-regulated in roots,but down-regulated in shoots by light.The results indicate that OsMPK14 could be implicated in diverse rice stimuli-responsive signaling cascades,and its expression might be regulated by multiple factors.