SYBR GreenⅠ实时PCR用于血小板抗原HPA-2、3基因分型的研究

被引：0

作者：

周世航 ^{[1
]}

梁晓华 ^{[1
]}

于卫建 ^{[1
]}

陈玫 ^{[1
]}

刘铭 ^{[2
]}

机构：

[1] 大连市血液中心血型研究室

[2] 大连医科大学细胞生物学教研室

来源：

临床血液学杂志 | 2014年 / 27卷 / 10期

关键词：

血小板抗原; 基因分型; PCR-SSP; 实时PCR;

D O I：

暂无

中图分类号：

R450 [];

学科分类号：

摘要：

目的:应用SYBR GreenⅠ实时PCR技术,建立一个血小板抗原HPA-2、3的基因分型方法。方法:应用SYBR GreenⅠ实时PCR,分别扩增样本的HPA-2、3的a和b等位基因。在PCR反应后,依据a和b等位基因Ct值的差值△Ct,使用聚类分析K-means cluster分析法,确定HPA-2、3的基因型。将SYBR GreenⅠ实时PCR的基因分型结果和常规PCR-SSP法进行比较。此外对该法的重复性进行评价。结果:105个血液样本的SYBR GreenⅠ实时PCR基因分型结果和常规PCR-SSP法完全一致。10个血样的2次SYBR GreenⅠ实时PCR基因分型结果是一致的。结论:HPA的SYBR GreenⅠ实时PCR分型方法具有快速、简单、准确、通量高、重复性好等优点,要优于常规的PCR-SSP法。

引用

页码：825 / 828

页数：4

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