Generation of a control induced pluripotent stem cell line (CBRCULi014-A) derived from the lymphoblastoid cells of a pediatric individual

被引:0
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作者
De Serres-Berard, Thiery [1 ]
Pouliot, Valerie [1 ]
Puymirat, Jack [2 ,3 ]
Chahine, Mohamed [1 ,3 ]
机构
[1] Inst Univ St Mentale Quebec, CERVO Brain Res Ctr, Quebec City, PQ G1J 2G3, Canada
[2] Univ Laval, CHU Quebec, LOEX, Res Ctr, Quebec City, PQ G1J 1Z4, Canada
[3] Univ Laval, Fac Med, Dept Med, Quebec City G1V 06A, PQ, Canada
关键词
D O I
10.1016/j.scr.2024.103587
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Lymphoblastoid cell lines serve as a readily and continuous resource for generating induced pluripotent stem cells (iPSCs), enabling the modeling of various genetic disorders in vitro. When investigating congenital and infantile diseases, age-matched controls derived from pediatric individuals are typically necessary, yet they may be scarce or difficult to obtain. Here, the Sendai virus system was employed to introduce reprogramming factors into lymphoblastoid cells derived from an apparently healthy 4-year-old female. The generated iPSCs strongly expressed pluripotency cell markers and displayed robust trilineage differentiation. CBRCULi014-A is therefore a reliable control iPSC line for pediatric disease investigation.
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页数:5
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