Investigating the Structure of the Components of the PolyADP-Ribosylation System in Fusarium Fungi and Evaluating the Expression Dynamics of Its Key Genes

被引：0

作者：

Stakheev, A. A. ^{[1
]}

Kutukov, R. R. ^{[1
]}

Taliansky, M. E. ^{[1
]}

Zavriev, S. K. ^{[1
]}

机构：

[1] Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia

来源：

ACTA NATURAE | 2024年 / 16卷 / 03期

基金：

俄罗斯科学基金会;

关键词：

Fusarium; parylation; PARP; PARG; transcription regulation; mycotoxin; expression; ADP-RIBOSYLATION; POLY(ADP-RIBOSE); TRICHOTHECENES; BIOSYNTHESIS; NAD(+); PARP; FOOD; INVOLVEMENT; ACTIVATION; FUMONISINS;

D O I：

10.32607/actanaturae.27450

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Poly(ADP-ribose) polymerase (PARP) is the key enzyme in polyADP-ribosylation, one of the main post-translational modifications. This enzyme is abundant in eukaryotic organisms. However, information on the PARP structure and its functions in members of the Fungi kingdom is very limited. In this study, we performed a bioinformatic search for homologs of PARP and its antagonist, PARG, in the genomes of four Fusarium strains using their whole-genome sequences annotated and deposited in databases. The F. graminearum PH-1, F. proliferatum ET-1, and F. oxysporum Fo47 strains were shown to possess a single homolog of both PARP and PARG. In addition, the F. oxysporum f. sp. lycopersici strain 4287 contained four additional proteins comprising PARP catalytic domains whose structure was different from that of the remaining identified homologs. Partial nucleotide sequences encoding the catalytic domains of the PARP and PARG homologs were determined in 11 strains of 9 Fusarium species deposited in all-Russian collections, and the phylogenetic properties of the analyzed genes were evaluated. In the toxigenic F. graminearum strain, we demonstrated up-regulation of the gene encoding the PARP homolog upon culturing under conditions stim- ulating the production of the DON mycotoxin, as well as up-regulation of the gene encoding PARG at later stages of growth. These findings indirectly indicate involvement of the polyADP-ribosylation system in the regulation of the genes responsible for DON biosynthesis.

引用

页数：112