Hurdle Approach to Simulate Corn Wet Milling Inactivation of Undesirable Microorganisms: A Pilot Scale Microbial Challenge Study Using Salmonella Surrogate Enterococcus faecium

被引：0

作者：

Kealey, Erin ^{[1
]}

Shick, Madeline ^{[1
]}

Kim, Minho ^{[1
]}

Chav, Ruben ^{[1
]}

Shetley, Gordon ^{[2
]}

Stenger, David ^{[3
]}

Perreau, Kirk A. ^{[4
]}

Cooke, Allison ^{[5
]}

Barnett-Neefs, Cecil ^{[1
]}

Stasiewicz, Matthew J. ^{[1
]}

机构：

[1] Univ Illinois, Dept Food Sci & Human Nutr, Urbana, IL 61801 USA

[2] Ingred Inc, Westchester, IL 60154 USA

[3] ADM, Decatur, IL 62526 USA

[4] Cargill Inc, Cedar Rapids, IA 52401 USA

[5] Corn Refiners Assoc, WASHINGTON, MD 20006 USA

来源：

JOURNAL OF FOOD PROTECTION | 2025年 / 88卷 / 01期

关键词：

Corn Starch; Hurdle; Hydrogen peroxide; Ring dryer; Sulfur dioxide; NRRL B-2354; ENTERICA;

D O I：

10.1016/j.jfp.2024.100432

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Corn wet milling (CWM) and corn starch flash drying processing conditions reduce undesirable microorganisms, such as Salmonella. Finished products are historically safe, with intrinsic properties such as low water activity inhibiting microbial growth. Corn processors could use quantified levels of reduction in this study of Salmonella surrogate Enterococcus faecium (E. faecium) to update their food safety plans. Industry-relevant conditions for CWM processes were recreated at pilot or lab scale for 3 unit operations: (1) steeping treatment in sulfur dioxide (SO2) with low (750 ppm SO2, 20 h, 43.3 degrees C), medium (1,500 ppm SO2, 30 h, 48.9 degrees C), and high (2,200 ppm SO2, 40 h, 53.3 degrees C) treatment conditions; (2) hydrogen peroxide (H2O2) treatment tested on bench scale with a factorial design (pH 3.5, 4.0, and 4.5), H2O2 concentrations (0.05%, 0.10%, 0.15% (w/w)), and temperatures (32, 38, and 46 degrees C) for 3 and 6 h; (3) flash drying treatment at 4 different temperatures (149, 177, 204, and 232 degrees C) with 2 different inoculation methods. E. faecium was reduced during each of these unit operations. By the end of each steeping treatment, E. faecium was consistently below the limit of quantitation (LOQ), meaning >6.5 log CFU/mL reduction in steep water, and >3.7 log CFU/g reduction in ground corn. The peroxide step had a reduction range from 0.03 log CFU/mL in the control group (0% H2O2 added) to >6 log CFU/mL observed in the high-intensity treatment of corn starch slurry. Flash drying had a reduction range from 1.7 to 2.7 log CFU/g. There was also no biologically meaningful change (<1 log CFU/g reduction) of E. faecium counts during an 8-week survival study of the dried final product. This hurdle approach study shows that existing CWM conditions are effective for Salmonella surrogate reduction through processing into finished starch and provides quantified E. faecium reductions for use in food safety plans.

引用

页数：9